Tinte iFluor® 405 succinimidyl ester

iFluor® 350 SE es razonablemente estable y muestra buena reactividad y selectividad con los grupos amino de proteínas.


Los tintes iFluor® de AAT Bioquest están optimizados para marcar proteínas, en particular, anticuerpos. Estos tintes son brillantes, fotoestables y tienen un enfriamiento mínimo de las proteínas. Pueden excitarse bien con las principales líneas láser de los instrumentos de fluorescencia (ejem. 350, 405, 488, 555 y 633 nm).

Los tintes iFluor® 405 tienen un máximo de excitación y emisión de fluorescencia de ~406 nm y ~427 nm respectivamente. Estas características espectrales los convierten en una excelente alternativa al colorante de etiquetado AMCA y Alexa Fluor® 405 (Alexa Fluor® es la marca registrada de Invitrogen).

iFluor® 405 SE es razonablemente estable y muestra buena reactividad y selectividad con los grupos amino de proteínas.

AAT-1021iFluor® 405 succinimidyl ester1mg
AAT-71021iFluor® 405 succinimidyl ester100 ug
AAT-71501iFluor® 405 succinimidyl ester5mg
AAT-71551iFluor® 405 succinimidyl ester10mg

Importante: Solo para uso en investigación (RUO). Almacenamiento: Congelación (< -15 °C). Minimizar la exposición a la luz.

Propiedades fisicas

Peso Molecular 755.58


Abrir en Advanced Spectrum Viewer

Propiedades espectrales

Factor de corrección (260 nm)0.48
Factor de corrección (280 nm)0.77
Coeficiente de extinción (cm -1 M -1)370001
Excitación (nm)403
Emisión (nm)427
Rendimiento cuántico0.911
1 Buffer acuoso (pH 7,2)


Preparación de la solución de stock común

Volumen de DMSO necesario para reconstituir la masa específica de succinimidil éster iFluor® 405 a la concentración dada. Tenga en cuenta que el volumen es solo para preparar la solución madre. Consulte el protocolo experimental de la muestra para conocer los buffers experimentales/fisiológicos apropiados.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM132.349 µL661.743 µL1.323 mL6.617 mL13.235 mL
5 mM26.47 µL132.349 µL264.697 µL1.323 mL2.647 mL
10 mM13.235 µL66.174 µL132.349 µL661.743 µL1.323 mL


Figura 1. Las células HL-60 se incubaron con (rojo, +) o sin (verde, -) anti-HLA-ABC humano (W6/32 mAb), seguido de iFluor® 405 cabra anti-ratón IgG (H&L). La señal de fluorescencia se controló utilizando un citómetro de flujo ACEA NovoCyte en el canal Pacific Blue (Ex/Em=405/445 nm).

Productos Similares

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
iFluor® 350 succinimidyl ester3454502000010.9510.830.23
iFluor® 488 succinimidyl ester4915167500010.910.210.11
iFluor® 514 succinimidyl ester5115277500010.8310.2650.116
iFluor® 532 succinimidyl ester5375609000010.6810.260.16
iFluor® 555 succinimidyl ester55757010000010.6410.230.14
iFluor® 594 succinimidyl ester58860418000010.5310.050.04
iFluor® 633 succinimidyl ester64065425000010.2910.0620.044
iFluor® 647 succinimidyl ester65667025000010.2510.030.03
iFluor® 660 succinimidyl ester66367825000010.2610.070.08
iFluor® 405 amine
iFluor® 405 maleimide
iFluor™ 405 azide
iFluor® 405 hydrazide
iFluor® A7 SE
iFluor® 350 maleimide
iFluor® 488 maleimide
iFluor® 555 maleimide
iFluor® 647 maleimide
iFluor® 680 maleimide
iFluor® 700 maleimide
iFluor® 750 maleimide
iFluor® 350 amine
iFluor® 488 amine
iFluor® 555 amine
iFluor® 647 amine
iFluor® 660 amine
iFluor® 680 amine
iFluor® 700 amine
iFluor® 710 amine
iFluor® 750 amine
iFluor® 350 hydrazide
iFluor® 488 hydrazide
iFluor® 555 hydrazide
iFluor® 647 hydrazide
iFluor® 680 hydrazide
iFluor® 700 hydrazide


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Application Notes

iFluor® Dye Selection Guide
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)


What are common laser lines used in flow cytometry?
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Are any of the cyanine dyes infrared?
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