Tinte iFluor® 350 succinimidyl ester

iFluor® 350 SE es razonablemente estable y muestra buena reactividad y selectividad con los grupos amino de proteínas.

Descripción

Los tintes iFluor® de AAT Bioquest están optimizados para marcar proteínas, en particular, anticuerpos. Estos tintes son brillantes, fotoestables y tienen un enfriamiento mínimo de las proteínas. Pueden excitarse bien con las principales líneas láser de los instrumentos de fluorescencia (p. ej., 350, 405, 488, 555 y 633 nm).

Los tintes iFluor® 350 tienen un máximo de excitación y emisión de fluorescencia de ~345 nm y ~450 nm respectivamente. Estas características espectrales los convierten en una excelente alternativa al colorante de etiquetado AMCA y Alexa Fluor® 350 (Alexa Fluor® es la marca registrada de Invitrogen).

iFluor® 350 SE es razonablemente estable y muestra buena reactividad y selectividad con los grupos amino de proteínas.

CatalogoProductoPresentación
AAT-1020iFluor® 350 succinimidyl ester1mg
AAT-71020iFluor® 350 succinimidyl ester100 ug
AAT-71500iFluor® 350 succinimidyl ester5mg
AAT-71550iFluor® 350 succinimidyl ester10mg

Importante: Solo para uso en investigación (RUO). Almacenamiento: Congelación (< -15 °C). Minimizar la exposición a la luz.

Propiedades fisicas

Peso Molecular 749.85
DisolventeDMSO

Espectro

Abrir en Advanced Spectrum Viewer

Propiedades espectrales

Factor de corrección (260 nm)0.83
Factor de corrección (280 nm)0.23
Coeficiente de extinción (cm -1 M -1)200001
Excitación (nm)345
Emisión (nm)450
Rendimiento cuántico0.951
1 Buffer acuoso (pH 7,2)

Calculadora

Preparación de la solución de stock común

Volumen de DMSO necesario para reconstituir la masa específica de succinimidil éster iFluor® 350 a la concentración dada. Tenga en cuenta que el volumen es solo para preparar la solución madre. Consulte el protocolo experimental de la muestra para conocer los buffers experimentales/fisiológicos apropiados.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM133.36 µL666.8 µL1.334 mL6.668 mL13.336 mL
5 mM26.672 µL133.36 µL266.72 µL1.334 mL2.667 mL
10 mM13.336 µL66.68 µL133.36 µL666.8 µL1.334 mL

Imagenes

Figura 1. Las células HeLa se tiñeron con antitubulina de conejo seguido de iFluor 350 de cabra anti-IgG de raton (H+L).

Productos Similares

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
iFluor® 405 succinimidyl ester4034273700010.9110.480.77
iFluor® 488 succinimidyl ester4915167500010.910.210.11
iFluor® 514 succinimidyl ester5115277500010.8310.2650.116
iFluor® 532 succinimidyl ester5375609000010.6810.260.16
iFluor® 555 succinimidyl ester55757010000010.6410.230.14
iFluor® 594 succinimidyl ester58860418000010.5310.050.04
iFluor® 633 succinimidyl ester64065425000010.2910.0620.044
iFluor® 647 succinimidyl ester65667025000010.2510.030.03
iFluor® 660 succinimidyl ester66367825000010.2610.070.08

Bibliografía

Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification
Authors: Gao, D and Liu, N and Li, Y and Zhang, Y and Liu, G and others, undefined
Journal: Metabolomics (Los Angel) (2017): 2153–0769

Suramin inhibits cullin-RING E3 ubiquitin ligases
Authors: Wu, Kenneth and Chong, Robert A and Yu, Qing and Bai, Jin and Spratt, Donald E and Ching, Kevin and Lee, Chan and Miao, Haibin and Tappin, Inger and Hurwitz, Jerard and others, undefined
Journal: Proceedings of the National Academy of Sciences (2016): E2011–E2018

Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function
Authors: Piccard, Helene and Berghmans, Nele and Korpos, Eva and Dillen, Chris and Aelst, Ilse Van and Li, S and ra , undefined and Martens, Erik and Liekens, S and ra , undefined and Noppen, Sam and Damme, Jo Van and others, undefined
Journal: International Journal of Cancer (2012): E425–E436

Referencias

Ver todas las 49 referencias: Citation Explorer

Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes
Authors: Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.
Journal: Mol Biosyst (2011): 1766

Visualizing dengue virus through Alexa Fluor labeling
Authors: Zhang S, Tan HC, Ooi EE.
Journal: J Vis Exp. (2011)

Fluorescent “Turn-on” system utilizing a quencher-conjugated peptide for specific protein labeling of living cells
Authors: Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.
Journal: Biochem Biophys Res Commun (2011): 211

Neuroanatomical basis of clinical joint application of “Jinggu” (BL 64, a source-acupoint) and “Dazhong” (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594
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Journal: Zhen Ci Yan Jiu (2011): 262

Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers
Authors: Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.
Journal: J Microbiol Methods (2011): 119

Alexa Fluor 546-ArIB[V11L;V16A] is a potent ligand for selectively labeling alpha 7 nicotinic acetylcholine receptors
Authors: Hone AJ, Whiteaker P, Mohn JL, Jacob MH, McIntosh JM.
Journal: J Neurochem (2010): 994

Asymmetric trimethine 3H-indocyanine dyes: efficient synthesis and protein labeling
Authors: Song F, Wang L, Qiao X, Wang B, Sun S, Fan J, Zhang L, Peng X.
Journal: Org Biomol Chem (2010): 4249

Neuroanatomical characteristics of acupoint “Chengshan” (BL 57) in the rat: a cholera toxin subunit B conjugated with Alexa Fluor 488 method study
Authors: Zhu XL, Bai WZ, Wu FD, Jiang J, Jing XH.
Journal: Zhen Ci Yan Jiu (2010): 433

Photoactivatable and photoconvertible fluorescent probes for protein labeling
Authors: Maurel D, Banala S, Laroche T, Johnsson K.
Journal: ACS Chem Biol (2010): 507

Novel Alexa Fluor-488 labeled antagonist of the A(2A) adenosine receptor: Application to a fluorescence polarization-based receptor binding assay
Authors: Kecskes M, Kumar TS, Yoo L, Gao ZG, Jacobson KA.
Journal: Biochem Pharmacol (2010): 506

Application Notes

iFluor® Dye Selection Guide
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)

FAQ

Is there a better dye than AMCA?
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Are any of the cyanine dyes infrared?
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