Tide Quencher™ 3 CPG [TQ3 CPG] *1000 Å* 

Este producto TQ3 se utiliza principalmente para el marcaje de oligonucleótidos durante la síntesis.

Descripción

TQ3 está diseñado para ser un extintor superior a TAMRA, TF3 y Cy3. TQ3 tiene (a). absorción mucho más fuerte; (b). mayor eficiencia de extinción; y c). formas reactivas versátiles con la solubilidad deseada para marcar oligonucleótidos y péptidos. El producto TQ3 se utiliza principalmente para el marcaje de oligonucleótidos durante la síntesis.

CatalogoProductoPresentación
AAT-2224Tide Quencher™ 3 CPG [TQ3 CPG] *1000 Å*100 mg

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Importante: Solo para uso en investigación (RUO). Almacenamiento: Congelación (< -15 °C). Minimizar la exposición a la luz.

Propiedades físicas

Peso Molecular N/D
DisolventeMeCN

Espectro

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Propiedades espectrales

Absorbancia573
Factor de corrección (260 nm)0.085
Factor de corrección (280 nm)0.091
Coeficiente de extinción (cm -1 M -1)22000

Imagen

Figura 1.

La síntesis de oligonucleótidos se lleva a cabo mediante la adición gradual de residuos de nucleótidos al 5′-terminus de la cadena en crecimiento hasta que se ensambla la secuencia deseada. Cada adición se denomina ciclo sintético y consta de cuatro reacciones químicas: desbloqueo (destritilación), acoplamiento, tapado y oxidación.

Productos Relacionados

NombreExtinction coefficient (cm -1 M -1)Correction Factor (260 nm)Correction Factor (280 nm)
Tide Quencher™ 4 CPG [TQ4 CPG] *500 Å*230000.1460.183
Tide Quencher™ 4 CPG [TQ4 CPG] *1000 Å*230000.1460.183
Tide Quencher™ 5 CPG [TQ5 CPG] *500 Å*
Tide Quencher™ 5 CPG [TQ5 CPG] *1000 Å*
Tide Quencher™ 1 CPG [TQ1 CPG] *500 Å*200000.1470.194
Tide Quencher™ 1 CPG [TQ1 CPG] *1000 Å*200000.1470.194
Tide Quencher™ 2 acid [TQ2 acid]210000.1000.12
Tide Quencher™ 2 amine [TQ2 amine]210000.1000.12
Tide Quencher™ 3 acid [TQ3 acid]220000.0850.091
Tide Quencher™ 2WS acid [TQ2WS acid]
Tide Quencher™ 2WS succinimidyl ester [TQ2WS, SE]
Tide Quencher™ 2WS maleimide [TQ2WS maleimide]
Tide Quencher™ 4WS acid [TQ4WS acid]
Tide Quencher™ 4WS amine [TQ4WS amine]
Tide Quencher™ 4WS maleimide [TQ4WS maleimide]
Tide Quencher™ 4WS succinimidyl ester [TQ4WS SE]
Tide Quencher™ 4WS azide [TQ4WS azide]
Tide Quencher™ 4WS alkyne [TQ4WS alkyne]
Tide Quencher™ 5WS acid [TQ5WS acid]

Bibliografía

A mechanistic model to predict effects of cathepsin B and cystatin C on β-amyloid aggregation and degradation
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Journal: Journal of Biological Chemistry (2017): jbc–M117

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Development of SNAP-Tag Fluorogenic Probes for Wash-Free Fluorescence Imaging
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FERRAMENTAS PARA ESTUDO DA BIOLOGIA DE GPCRS (G-PROTEIN COUPLED RECEPTORS)
Authors: Soriani, Frederico Marianetti and Russo, Remo Castro

Referencias

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Journal: Bioconjug Chem (2009): 147

The detection of platelet derived growth factor using decoupling of quencher-oligonucleotide from aptamer/quantum dot bioconjugates
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Development of a cell-based hepatitis C virus infection fluorescent resonance energy transfer assay for high-throughput antiviral compound screening
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An improved cell-penetrating, caspase-activatable, near-infrared fluorescent peptide for apoptosis imaging
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Feasibility of single nucleotide polymorphism genotyping with a single-probe by time-resolved Forster resonance energy transfer
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Photodynamic molecular beacon triggered by fibroblast activation protein on cancer-associated fibroblasts for diagnosis and treatment of epithelial cancers
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Application Notes (en Ingles)

A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Abbreviation of Common Chemical Compounds Related to Peptides
Annexin V