Texas Red-X, succinimidyl ester *Single isomer*   

Este reactivo con amina succinimidil éster Texas Red®-X, es un reemplazo superior al Texas Red® (marca registrada de Invitrogen).


Este reactivo con amina succinimidil éster Texas Red®-X, es un reemplazo superior al Texas Red (Texas Red® es la marca registrada de Invitrogen). Tiene las mismas propiedades espectrales que Texas Red, pero es mucho más fácil de conjugar con una biomolécula con un rendimiento de conjugación mucho mayor. Se puede utilizar para crear bioconjugados fluorescentes de color rojo brillante con un máximo de excitación/emisión de ~595/615 nm. En comparación con Texas Red®, este colorante reactivo contiene un espaciador de aminohexanoilo (“X”) adicional de siete átomos entre el fluoróforo y el grupo éster de succinimidilo. Este espaciador ayuda a separar el fluoróforo de su punto de unión, reduciendo potencialmente la interacción del fluoróforo con la biomolécula a la que está conjugado. En comparación con los isómeros mixtos (#474), este producto es el isómero 5 único, lo que brinda resultados más reproducibles.

AAT-474Texas Red-X, succinimidyl ester *Single isomer* *178623-11-5*5mg

Importante: Solo para uso en investigación (RUO). Almacenamiento: Congelación (< -15 °C). Minimizar la exposición a la luz.

Propiedades fisicas

Peso Molecular 816.94


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Propiedades espectrales

Factor de corrección (260 nm)0.23
Factor de corrección (280 nm)0.36
Factor de corrección (565 nm)0.4472
Coeficiente de extinción (cm -1 M -1)1160001
Excitación (nm)586
Emisión (nm)603
Rendimiento cuántico0.932, 0.351
1 Agua, 2 Etanol,


Preparación de la solución de stock común

Volumen de DMSO necesario para reconstituir la masa específica de Texas Red-X, succinimidil éster Isómero único 178623-11-5 a la concentración dada. Tenga en cuenta que el volumen es solo para preparar la solución madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales/fisiológicos apropiados.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM122.408 µL612.04 µL1.224 mL6.12 mL12.241 mL
5 mM24.482 µL122.408 µL244.816 µL1.224 mL2.448 mL
10 mM12.241 µL61.204 µL122.408 µL612.04 µL1.224 mL


Figura 1. Estructura quimica de Texas Red-X, succinimidyl ester *Single isomer* *CAS 178623-11-5*

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Application Notes (en Ingles)

A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)