La sonda Dihidrorodamina 123 se puede utilizar para mediciones multiplex. No para uso diagnóstico o terapéutico.
Descripción
La dihidrorodamina 123 es, por mucho, la sonda más utilizada para medir el H2O2 intracelular. DHR 123 se oxida directamente a rodamina 123, que es excitable a 488 y emite a 515 nm en el mismo rango de emisión que FITC.
Es ampliamente utilizado en neutrófilos humanos, eosinófilos humanos, Células HL60, mastocitos de rata, neutrófilos de cobayo, condrocitos cultivados, cerebro de rata, células tubulares proximales renales de rata, células mesangiales y L929 células. En combinación con otros reactivos fluorescentes (como análisis de receptores de superficie por anticuerpos fluorescentes, viabilidad celular usando propidio indicadores de yoduro y calcio), esta sonda se puede utilizar para mediciones multiplex.
Nombre en Ingles: Dihydrorhodamine 123 *CAS 109244-58-8*
| Catalogo | Producto | Presentación |
|---|---|---|
| AAT-15206 | Dihidrorodamina 123 CAS 109244-58-8 | 10 mg |
| AAT-15207 | Dihidrorodamina 123 CAS 109244-58-8 | 5 x 1mg |
Importante: Producto no diseñado para uso diagnóstico o terapéutico. Solo para uso en investigación (RUO). Almacenamiento a largo plazo: Congelacion a < -15 °C. Minimizar la exposición a la luz.
Propiedades Fisicas
| Peso Molecular | 346.38 |
| Disolvente | DMSO |
Espectro
Abrir en Advanced Spectrum Viewer
Propiedades Espectrales
| Absorbancia (nm) | 497 |
| Coeficiente de extinción (cm -1 M -1) | 85200 |
| Excitación (nm) | 508 |
| Emisión (nm) | 528 |
Calculadora
Preparación de la solución de stock común
Volumen de DMSO necesario para reconstituir la masa específica de Dihydrorhodamine 123 CAS 109244-58-8 a la concentración dada. Tenga en cuenta que el volumen es solo para preparar la solución madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales/fisiológicos apropiados.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 288.7 µL | 1.444 mL | 2.887 mL | 14.435 mL | 28.87 mL |
| 5 mM | 57.74 µL | 288.7 µL | 577.401 µL | 2.887 mL | 5.774 mL |
| 10 mM | 28.87 µL | 144.35 µL | 288.7 µL | 1.444 mL | 2.887 mL |
Figura 1. Estructura quimica para Dihydrorhodamine 123 *CAS 109244-58-8*
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| Rhodamine 123 *CAS 62669-70-9* | 508 | 528 | 85200 |
Informacion Importante
Este prodcuto no está diseñado para uso alimentario, farmacéutico, doméstico, agrícola o cosmético. Su uso debe ser supervisado por un persona técnicamente calificada con experiencia en el manejo de productos químicos. Lo anterior se cree información correcta, pero no pretende ser unica ni completa y se utilizará solo como una guía.
Productos Adicionales
Bibliografias
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Liver necrosis and lethal systemic inflammation in a murine model of Rickettsia typhi infection: role of neutrophils, macrophages and NK cells
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Journal: PLoS Negl Trop Dis (2016): e0004935
Charakterisierung angeborener Immunmechanismen und immunpathologischer Effekte in der Rickettsia typhi-Infektion (Wolbach und Todd, 1920) in der Maus (Mus musculus, Linnaeus, 1758)
Authors: Papp, Stefanie
Journal: (2015)
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Journal: (2013)
Bicarbonate plays a critical role in the generation of cytotoxicity during SIN-1 decomposition in culture medium
Authors: Shirai, Kyo and Okada, Tatsumi and Konishi, Kanako and Murata, Hiroshi and Akashi, Soichiro and Sugawara, Fumio and Watanabe, Nobuo and Arai, Takao
Journal: Oxidative medicine and cellular longevity (2012)
Direct Macromolecular Drug Delivery to Cerebral Ischemia Area using Neutrophil-Mediated Nanoparticles
Authors: Zhang, Chun and Ling, Cheng-li and Pang, Liang and Wang, Qi and Liu, Jing-xin and Wang, Bing-shan and Liang, Jian-ming and Guo, Yi-zhen and Qin, Jing and Wang, Jian-xin
Referencias
Ver todas las 20 referencias: Citation Explorer
Chronic granulomatous disease (CGD) and complete myeloperoxidase deficiency both yield strongly reduced dihydrorhodamine 123 test signals but can be easily discerned in routine testing for CGD
Authors: Mauch L, Lun A, O’Gorman MR, Harris JS, Schulze I, Zychlinsky A, Fuchs T, Oelschlagel U, Brenner S, Kutter D, Rosen-Wolff A, Roesler J.
Journal: Clin Chem (2007): 890
Reactivity of 2′,7′-dichlorodihydrofluorescein and dihydrorhodamine 123 and their oxidized forms toward carbonate, nitrogen dioxide, and hydroxyl radicals
Authors: Wrona M, Patel K, Wardman P.
Journal: Free Radic Biol Med (2005): 262
Fluorescence measurements of steady state peroxynitrite production upon SIN-1 decomposition: NADH versus dihydrodichlorofluorescein and dihydrorhodamine 123
Authors: Martin-Romero FJ, Gutierrez-Martin Y, Henao F, Gutierrez-Merino C.
Journal: J Fluoresc (2004): 17
Diagnostic paradigm for evaluation of male patients with chronic granulomatous disease, based on the dihydrorhodamine 123 assay
Authors: Jirapongsananuruk O, Malech HL, Kuhns DB, Niemela JE, Brown MR, Anderson-Cohen M, Fleisher TA.
Journal: J Allergy Clin Immunol (2003): 374
Detection of reactive nitrogen species using 2,7-dichlorodihydrofluorescein and dihydrorhodamine 123
Authors: Ischiropoulos H, Gow A, Thom SR, Kooy NW, Royall JA, Crow JP.
Journal: Methods Enzymol (1999): 367
Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: comparison with 2′,7′-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2′,7′-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123
Authors: Hempel SL, Buettner GR, O’Malley YQ, Wessels DA, Flaherty DM.
Journal: Free Radic Biol Med (1999): 146
Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: implications for intracellular measurement of reactive nitrogen and oxygen species
Authors: Crow JP., undefined
Journal: Nitric Oxide (1997): 145
Limitations on the use of dihydrorhodamine 123 for flow cytometric analysis of the neutrophil respiratory burst
Authors: van Pelt LJ, van Zwieten R, Weening RS, Roos D, Verhoeven AJ, Bolscher BG.
Journal: J Immunol Methods (1996): 187
Dihydrorhodamine 123 identifies impaired mitochondrial respiratory chain function in cultured cells harboring mitochondrial DNA mutations
Authors: Sobreira C, Davidson M, King MP, Mir and a AF., undefined
Journal: J Histochem Cytochem (1996): 571
Comparative use of 2,7-dichlorofluorescein diacetate, dihydrorhodamine 123, and hydroethidine for studying oxidative metabolism of phagocytosing cells
Authors: Biziukin AV, Korkina LG, Velichkovskii BT.
Journal: Biull Eksp Biol Med (1995): 361
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