Kit de ensayo Cell Meter™ de apoptosis de fosfatidilserina

apoptosis

Kit de ensayo Cell Meter™ de apoptosis de fosfatidilserina (Fluorescencia verde) está diseñado para monitorear la apoptosis celular midiendo la translocación de fosfatidilserina (PS).

Descripción

Kit de ensayo Cell Meter™ de apoptosis de fosfatidilserina, Fluorescencia verde optimizada para lectores de microplacas

Los kits de ensayo Cell Meter™ son un conjunto de herramientas para monitorear la viabilidad celular. Existe una variedad de parámetros que se pueden utilizar para monitorear la viabilidad celular.

Este kit en particular está diseñado para monitorear la apoptosis celular midiendo la translocación de fosfatidilserina (PS). En la apoptosis, la PS se transfiere a la valva externa de la membrana plasmática. La aparición de fosfatidilserina en la superficie celular es un indicador universal de las etapas inicial/intermedia de la apoptosis celular y puede detectarse antes de que se puedan observar cambios morfológicos.

El kit está diseñado para monitorear la apoptosis celular midiendo la translocación de fosfatidilserina (PS), y utiliza nuestro sensor de PS Apopxin™ basado en moléculas pequeñas fluorescentes patentado que se une específicamente a PS con una afinidad mucho mayor que la anexina V (Kd < 10 nM). Tiene fluorescencia verde al unirse a la membrana PS. Se puede utilizar en formatos de microplaca, microscopio y citómetro de flujo, mientras que la mayoría de los demás kits de ensayo de apoptosis comerciales solo se utilizan con microscopio o plataforma de citometría de flujo.

Nombre en Ingles: Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Green Fluorescence Optimized for Microplate Readers*

CatalogoProductoPresentación
AAT-22791Kit de ensayo Cell Meter™ de apoptosis de fosfatidilserina100 pruebas

Componentes

Component A: Apopxin™ Green (100X Stock Solution)1 vial (100 µL/vial)
Component B: Assay Buffer (4 °C)1 frasco (10 mL)

Importante, Solo para uso en investigación (RUO). Almacenamiento: Refrigeración de 2-8 °C. Minimizar la exposición a la luz.

Plataforma

Lector de fluorescencia de microplacas

Excitación490 nm
Emisión525 nm
Cutoff515 nm
Placa recomendadaPared negra, fondo claro
Especificaciones InstrumentoModo lectura inferior

Imagenes

Figura 1. Detección de actividad de unión a fosfatidilserina en células Jurkat. Se sembraron células Jurkat el mismo día a razón de 200.000 células/90 µl/pocillo en una placa de 96 pocillos de pared negra/fondo transparente Costar. Las células se trataron con diferentes dosis de camptotecina durante 5 horas como se indica. Se añadió la solución de ensayo Apopxin™ Green (100 µl/pocillo) y se incubó a temperatura ambiente durante 1 hora. La intensidad de la fluorescencia se midió a Ex/Em = 490/525 nm con el instrumento NOVOstar (de BMG Labtech) usando el modo de lectura inferior.

Figura 1: Imágenes de células Jurkat teñidas con el kit de ensayo de apoptosis de fosfatidilserina Cell Meter™ en una placa de 96 pocillos de pared negra/fondo transparente Costar. A: Células de control no tratadas. B: Células tratadas con camptotecina 20 µM durante 5 horas.

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Formatos Alternativos

Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Green Fluorescence Optimized for Microplate Readers*
Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Orange Fluorescence Optimized for Microplate Readers*
Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Red Fluorescence Optimized for Microplate Readers*
Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Deep Red Fluorescence Optimized for Microplate Readers*
Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Green Fluorescence Optimized for Flow Cytometry*
Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Deep Red Fluorescence Optimized for Flow Cytometry*
Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Blue Fluorescence Excited at 405 nm*
Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Green Fluorescence Excited at 405 nm*

Bibliografia

STE20-Type Kinases MST3 and MST4 Act Non-Redundantly to Promote the Progression of Hepatocellular Carcinoma
Authors: Caputo, Mara and Xia, Ying and Anand, Sumit Kumar and Cansby, Emmelie and Andersson, Emma and Marschall, Hanns-Ulrich and K{\”o}nigsrainer, Alfred and Peter, Andreas and Mahlapuu, Margit
Journal: (2023)

Vascular endothelial growth factor on Runt-related transcript factor-2 in aortic valve cells
Authors: Li, Shao-Jung and Kao, Yu-Hsun and Chung, Cheng-Chih and Cheng, Wan-Li and Lin, Yung-Kuo and Chen, Yi-Jen
Journal: European Journal of Clinical Investigation (2020): e13470

Combined Treatments of Magnetic Intra-Lysosomal Hyperthermia with Doxorubicin Promotes Synergistic Anti-Tumoral Activity.
Authors: El, D Hajj Diab and Clerc, P and Serhan, N and Fourmy, D and Gigoux, V
Journal: Nanomaterials (Basel, Switzerland) (2018)

Integrin $\beta$3 inhibits hypoxia-induced apoptosis in cardiomyocytes
Authors: Su, Yifan and Tian, Hua and Wei, Lijiang and Fu, Guohui and Sun, Ting
Journal: Acta Biochimica et Biophysica Sinica (2018): 658–665

Tumor-selective mitochondrial network collapse induced by atmospheric gas plasma-activated medium.
Authors: Saito, Kosuke and Asai, Tomohiko and Fujiwara, Kyoko and Sahara, Junki and Koguchi, Haruhisa and Fukuda, Noboru and Suzuki-Karasaki, Miki and Soma, Masayoshi and Suzuki-Karasaki, Yoshihiro
Journal: Oncotarget (2016)

Physiological effects of the herbicide glyphosate on the cyanobacterium Microcystis aeruginosa
Authors: Wu, Liang and Qiu, Zhihao and Zhou, Ya and Du, Yuping and Liu, Chaonan and Ye, Jing and Hu, Xiaojun
Journal: Aquatic Toxicology (2016): 72–79

Inhibition of malignant phenotypes of human osteosarcoma cells by a gene silencer, a pyrrole–imidazole polyamide, which targets an E-box motif
Authors: Taniguchi, Masashi and Fujiwara, Kyoko and Nakai, Yuji and Ozaki, Toshinori and Koshikawa, Nobuko and Toshio, Kojima and Kataba, Motoaki and Oguni, Asako and Matsuda, Hiroyuki and Yoshida, Yukihiro and others, undefined
Journal: FEBS open bio (2014): 328–334

Referencias

Ver todas las 92 referencias: Citation Explorer

Suicidal membrane repair regulates phosphatidylserine externalization during apoptosis
Authors: Mirnikjoo B, Balasubramanian K, Schroit AJ.
Journal: J Biol Chem (2009): 22512

Peptidic targeting of phosphatidylserine for the MRI detection of apoptosis in atherosclerotic plaques
Authors: Burtea C, Laurent S, Lancelot E, Ballet S, Murariu O, Rousseaux O, Port M, V and er Elst L, Corot C, Muller RN.
Journal: Mol Pharm (2009): 1903

Detection of apoptosis based on the interaction between annexin V and phosphatidylserine
Authors: Liu T, Zhu W, Yang X, Chen L, Yang R, Hua Z, Li G.
Journal: Anal Chem (2009): 2410

Evaluation of cell surface expression of phosphatidylserine in ovarian carcinoma effusions using the annexin-V/7-AAD assay: clinical relevance and comparison with other apoptosis parameters
Authors: Dong HP, Holth A, Kleinberg L, Ruud MG, Elstr and MB, Trope CG, Davidson B, Risberg B.
Journal: Am J Clin Pathol (2009): 756

Mobilization of lysosomal calcium regulates the externalization of phosphatidylserine during apoptosis
Authors: Mirnikjoo B, Balasubramanian K, Schroit AJ.
Journal: J Biol Chem (2009): 6918

Trivalent methylated arsenical-induced phosphatidylserine exposure and apoptosis in platelets may lead to increased thrombus formation
Authors: Bae ON, Lim KM, Noh JY, Chung SM, Kim SH, Chung JH.
Journal: Toxicol Appl Pharmacol (2009): 144

Discovery of a phosphatidylserine-recognizing peptide and its utility in molecular imaging of tumour apoptosis
Authors: Thapa N, Kim S, So IS, Lee BH, Kwon IC, Choi K, Kim IS.
Journal: J Cell Mol Med (2008): 1649

Caspase-dependent and -independent induction of phosphatidylserine externalization during apoptosis in human renal carcinoma Cak(1)-1 and A-498 cells
Authors: Lock EA, Reed CJ, Kinsey GR, Schnellmann RG.
Journal: Toxicology (2007): 79

Regulated externalization of phosphatidylserine at the cell surface: implications for apoptosis
Authors: Balasubramanian K, Mirnikjoo B, Schroit AJ.
Journal: J Biol Chem (2007): 18357

PET imaging of apoptosis with (64)Cu-labeled streptavidin following pretargeting of phosphatidylserine with biotinylated annexin-V
Authors: Cauchon N, Langlois R, Rousseau JA, Tessier G, Cadorette J, Lecomte R, Hunting DJ, Pavan RA, Zeisler SK, van Lier JE.
Journal: Eur J Nucl Med Mol Imaging (2007): 247

Application notes

Abbreviation of Common Chemical Compounds Related to Peptides
Annexin V
Calcein
Experimental Protocol for Calcein AM Assay
Human High Temperature Requirement Serine Protease A1 (HTRA1) Degrades Tau Protein Aggregates

FAQ

Are inflammasomes and caspase-1 related?
Do you offer any fluorimetric assays that measure caspase activation/activity in live cells using a flow cytometer?
Does pH and staining temperature affect Annexin V-Phosphatidylserine binding?
Does propidium iodide stain apoptotic cells?
How can I tell if my cell sample is dying?

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