El indicador Calbryte 520XL tiene baja afinidad por el ion calcio con Kd ~300 uM, que es similar al conocido Rhod 5N, pero mucho más estable que Rhod-5N.
Descripción
El indicador Calbryte 520XL, AM es un nuevo indicador de calcio permeable a las células y fluorescente con una afinidad extremadamente baja. Al igual que otras cargas de células AM de tinte, el éster AM de Calbryte 520XL no es fluorescente y una vez que ingresa a la célula, es hidrolizado por la esterasa intracelular y se activa. El indicador activado es una molécula polar que ya no es capaz de difundirse libremente a través de la membrana celular, esencialmente atrapada dentro de las células.
Calbryte 520XL tiene baja afinidad por el ion calcio con Kd ~300 uM, que es similar al conocido Rhod 5N, pero mucho más estable que Rhod-5N. Calbryte 520XL produce una señal de fluorescencia brillante en presencia de iones de calcio en alta concentración. Tiene la misma longitud de onda de excitación y emisión que Fluo-4, por lo que la misma configuración de ensayo de Fluo-4 se puede aplicar fácilmente a los ensayos de calcio basados en Calbryte 520XL.
Calbryte 520XL es una excelente alternativa a Rhod-5N. También ofrecemos Calbryte 520XL, sal de potasio (n.º 20645), Calbryte 520XL-dextrano (n.º 20648), azida Calbryte 520XL (n.º 20643) que se pueden conjugar fácilmente con un vehículo a través de la conocida química de clic.
Nombre en ingles: Calbryte™-520XL AM
Importante: Solo para uso en investigación (RUO). Almacenamiento: Congelar a (< -15 °C), Minimizar la exposición a la luz.
| Catalogo | Producto | Presentación |
|---|---|---|
| AAT-20646 | Indicador Calbryte™-520XL AM | 10 x 50 ug |
Plataforma
Microscopio de Fluorescencia
| Excitación | FITC |
| Emisión | FITC |
| Placa Recomendada | Pared negra/Fondo claro |
Lector de microplacas de Fluorescencia
| Excitación | 490 |
| Emisión | 525 |
| Cutoff | 515 |
| Placa Recomendada | Pared Negra / fondo claro |
| Especificaciones Instrumento | Modo de lectura inferior / manipulación de liquidos programable |
Espectro
Abrir en Advanced Spectrum Viewer
Propiedades espectrales
| Excitación (nm) | 493 |
| Emisión (nm) | 515 |
| Rendimiento cuántico | 0.751 |
Calculadora
Volumen de DMSO necesario para reconstituir la masa específica de Calbryte™-520XL AM a la concentración dada. Tenga en cuenta que el volumen es solo para preparar la solución madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales/fisiológicos apropiados.
uffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 109.677 µL | 548.384 µL | 1.097 mL | 5.484 mL | 10.968 mL |
| 5 mM | 21.935 µL | 109.677 µL | 219.354 µL | 1.097 mL | 2.194 mL |
| 10 mM | 10.968 µL | 54.838 µL | 109.677 µL | 548.384 µL | 1.097 mL |
Imagen
Figura 1. Emisión de fluorescencia dependiente de Ca2+ del indicador Calbryte™-520XL (Ex/Em = 490/525 nm).
Productos Relacionados
| Name | Excitation (nm) | Emission (nm) | Quantum yield |
| Calbryte™ 520, potassium salt | 493 | 515 | 0.751 |
| Calbryte™ 590, potassium salt | 581 | 593 | – |
| Calbryte™ 630, potassium salt | 607 | 624 | – |
| Calbryte™-520XL, potassium salt | 493 | 515 | 0.751 |
Bibliografía
Ver todas las 13 bibliografias: Citation Explorer
Calreticulin regulates TGF-β1-induced epithelial mesenchymal transition through modulating Smad signaling and calcium signaling
Authors: Wu, Yanjiao and Xu, Xiaoli and Ma, Lunkun and Yi, Qian and Sun, Weichao and Tang, Liling
Journal: The International Journal of Biochemistry & Cell Biology (2017)
Monosialoganglioside 1 may alleviate neurotoxicity induced by propofol combined with remifentanil in neural stem cells
Authors: Lu, Jiang and Yao, Xue-qin and Luo, Xin and Wang, Yu and Chung, Sookja Kim and Tang, He-xin and Cheung, Chi Wai and Wang, Xian-yu and Meng, Chen and Li, Qing and others, undefined
Journal: Neural Regeneration Research (2017): 945
Obtaining spontaneously beating cardiomyocyte-like cells from adipose-derived stromal vascular fractions cultured on enzyme-crosslinked gelatin hydrogels
Authors: Yang, Gang and Xiao, Zhenghua and Ren, Xiaomei and Long, Haiyan and Ma, Kunlong and Qian, Hong and Guo, Yingqiang
Journal: Scientific Reports (2017): 41781
Dexmedetomidine reduces hypoxia/reoxygenation injury by regulating mitochondrial fission in rat hippocampal neurons
Authors: Liu, Jia and Du, Qing and Zhu, He and Li, Yu and Liu, Maodong and Yu, Shoushui and Wang, Shilei
Journal: Int J Clin Exp Med (2017): 6861–6868
Di (2-ethylhexyl) phthalate-induced apoptosis in rat INS-1 cells is dependent on activation of endoplasmic reticulum stress and suppression of antioxidant protection
Authors: Sun, Xia and Lin, Yi and Huang, Qiansheng and Shi, Junpeng and Qiu, Ling and Kang, Mei and Chen, Yajie and Fang, Chao and Ye, Ting and Dong, Sijun
Journal: Journal of cellular and molecular medicine (2015): 581–594
The effect of mitochondrial calcium uniporter on mitochondrial fission in hippocampus cells ischemia/reperfusion injury
Authors: Zhao, Lantao and Li, Shuhong and Wang, Shilei and Yu, Ning and Liu, Jia
Journal: Biochemical and biophysical research communications (2015): 537–542
Role of mitochondrial calcium uniporter in regulating mitochondrial fission in the cerebral cortexes of living rats
Authors: Liang, Nan and Wang, Peng and Wang, Shilei and Li, Shuhong and Li, Yu and Wang, Jinying and Wang, Min
Journal: Journal of Neural Transmission (2014): 593–600
Propofol and remifentanil at moderate and high concentrations affect proliferation and differentiation of neural stem/progenitor cells
Authors: Li, Qing and Lu, Jiang and Wang, Xianyu and others, undefined
Journal: Neural regeneration research (2014): 2002
Fungus induces the release of IL-8 in human corneal epithelial cells, via Dectin-1-mediated protein kinase C pathways.
Authors: Peng, Xu-Dong and Zhao, Gui-Qiu and Lin, Jing and Jiang, Nan and Xu, Qiang and Zhu, Cheng-Cheng and Qu, Jain-Qiu and Cong, Lin and Li, Hui
Journal: International journal of ophthalmology (2014): 441–447
Increased expression of cell adhesion molecule 1 by mast cells as a cause of enhanced nerve–mast cell interaction in a hapten-induced mouse model of atopic dermatitis
Authors: Hagiyama, M and Inoue, T and Furuno, T and Iino, T and Itami, S and Nakanishi, M and Asada, H and Hosokawa, Y and Ito, A
Journal: British Journal of Dermatology (2013): 771–778
Referencias
Ver todas las 53 referencias: Citation Explorer
A flow cytometric comparison of Indo-1 to fluo-3 and Fura Red excited with low power lasers for detecting Ca(2+) flux
Authors: Bailey S, Macardle PJ.
Journal: J Immunol Methods (2006): 220
Functional fluo-3/AM assay on P-glycoprotein transport activity in L1210/VCR cells by confocal microscopy
Authors: Orlicky J, Sulova Z, Dovinova I, Fiala R, Zahradnikova A, Jr., Breier A.
Journal: Gen Physiol Biophys (2004): 357
Comparison of human recombinant adenosine A2B receptor function assessed by Fluo-3-AM fluorometry and microphysiometry
Authors: Patel H, Porter RH, Palmer AM, Croucher MJ.
Journal: Br J Pharmacol (2003): 671
Measurement of the dissociation constant of Fluo-3 for Ca2+ in isolated rabbit cardiomyocytes using Ca2+ wave characteristics
Authors: Loughrey CM, MacEachern KE, Cooper J, Smith GL.
Journal: Cell Calcium (2003): 1
A sensitive method for the detection of foot and mouth disease virus by in situ hybridisation using biotin-labelled oligodeoxynucleotides and tyramide signal amplification
Authors: Zhang Z, Kitching P.
Journal: J Virol Methods (2000): 187
Kinetics of onset of mouse sperm acrosome reaction induced by solubilized zona pellucida: fluorimetric determination of loss of pH gradient between acrosomal lumen and medium monitored by dapoxyl (2-aminoethyl) sulfonamide and of intracellular Ca(2+) chang
Authors: Rockwell PL, Storey BT.
Journal: Mol Reprod Dev (2000): 335
MRP2, a human conjugate export pump, is present and transports fluo 3 into apical vacuoles of Hep G2 cells
Authors: Cantz T, Nies AT, Brom M, Hofmann AF, Keppler D.
Journal: Am J Physiol Gastrointest Liver Physiol (2000): G522
Use of co-loaded Fluo-3 and Fura Red fluorescent indicators for studying the cytosolic Ca(2+)concentrations distribution in living plant tissue
Authors: Walczysko P, Wagner E, Albrechtova JT.
Journal: Cell Calcium (2000): 23
[Ca2+]i following extrasystoles in guinea-pig trabeculae microinjected with fluo-3 – a comparison with frog skeletal muscle fibres
Authors: Wohlfart B., undefined
Journal: Acta Physiol Scand (2000): 1
Determination of the intracellular dissociation constant, K(D), of the fluo-3. Ca(2+) complex in mouse sperm for use in estimating intracellular Ca(2+) concentrations
Authors: Rockwell PL, Storey BT.
Journal: Mol Reprod Dev (1999): 418
Aplication Notes
A Comparison of Fluorescent Red Calcium Indicators for Detecting Intracellular Calcium Mobilization in CHO Cells
A Meta-Analysis of Common Calcium Indicators
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
FAQ
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AssayWise
Multicolor Intracellular Calcium Detection Probes
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