Indicador de calcio Calbryte™-520XL AM

indicador calbryte

El indicador Calbryte 520XL tiene baja afinidad por el ion calcio con Kd ~300 uM, que es similar al conocido Rhod 5N, pero mucho más estable que Rhod-5N.


El indicador Calbryte 520XL, AM es un nuevo indicador de calcio permeable a las células y fluorescente con una afinidad extremadamente baja. Al igual que otras cargas de células AM de tinte, el éster AM de Calbryte 520XL no es fluorescente y una vez que ingresa a la célula, es hidrolizado por la esterasa intracelular y se activa. El indicador activado es una molécula polar que ya no es capaz de difundirse libremente a través de la membrana celular, esencialmente atrapada dentro de las células.

Calbryte 520XL tiene baja afinidad por el ion calcio con Kd ~300 uM, que es similar al conocido Rhod 5N, pero mucho más estable que Rhod-5N. Calbryte 520XL produce una señal de fluorescencia brillante en presencia de iones de calcio en alta concentración. Tiene la misma longitud de onda de excitación y emisión que Fluo-4, por lo que la misma configuración de ensayo de Fluo-4 se puede aplicar fácilmente a los ensayos de calcio basados en Calbryte 520XL.

Calbryte 520XL es una excelente alternativa a Rhod-5N. También ofrecemos Calbryte 520XL, sal de potasio (n.º 20645), Calbryte 520XL-dextrano (n.º 20648), azida Calbryte 520XL (n.º 20643) que se pueden conjugar fácilmente con un vehículo a través de la conocida química de clic.

Nombre en ingles: Calbryte™-520XL AM

Importante: Solo para uso en investigación (RUO). Almacenamiento: Congelar a  (< -15 °C), Minimizar la exposición a la luz.

AAT-20646Indicador Calbryte™-520XL AM10 x 50 ug


Microscopio de Fluorescencia

Placa RecomendadaPared negra/Fondo claro

Lector de microplacas de Fluorescencia

Placa Recomendada Pared Negra / fondo claro
Especificaciones InstrumentoModo de lectura inferior / manipulación de liquidos programable


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Propiedades espectrales

Excitación (nm)493
Emisión (nm)515
Rendimiento cuántico0.751
1 Calcium bound (pH 7.2)


Volumen de DMSO necesario para reconstituir la masa específica de Calbryte™-520XL AM a la concentración dada. Tenga en cuenta que el volumen es solo para preparar la solución madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales/fisiológicos apropiados.


0.1 mg0.5 mg1 mg5 mg10 mg
1 mM109.677 µL548.384 µL1.097 mL5.484 mL10.968 mL
5 mM21.935 µL109.677 µL219.354 µL1.097 mL2.194 mL
10 mM10.968 µL54.838 µL109.677 µL548.384 µL1.097 mL


Figura 1. Emisión de fluorescencia dependiente de Ca2+ del indicador Calbryte™-520XL (Ex/Em = 490/525 nm).

Productos Relacionados

NameExcitation (nm)Emission (nm)Quantum yield
Calbryte™ 520, potassium salt4935150.751
Calbryte™ 590, potassium salt581593
Calbryte™ 630, potassium salt607624
Calbryte™-520XL, potassium salt4935150.751
1 Calcium bound (pH 7.2)

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Fungus induces the release of IL-8 in human corneal epithelial cells, via Dectin-1-mediated protein kinase C pathways.
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Increased expression of cell adhesion molecule 1 by mast cells as a cause of enhanced nerve–mast cell interaction in a hapten-induced mouse model of atopic dermatitis
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Aplication Notes

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