Indicador de calcio Calbryte™-520XL AM

indicador calbryte

El indicador Calbryte 520XL tiene baja afinidad por el ion calcio con Kd ~300 uM, que es similar al conocido Rhod 5N, pero mucho más estable que Rhod-5N.

Descripción

El indicador Calbryte 520XL, AM es un nuevo indicador de calcio permeable a las células y fluorescente con una afinidad extremadamente baja. Al igual que otras cargas de células AM de tinte, el éster AM de Calbryte 520XL no es fluorescente y una vez que ingresa a la célula, es hidrolizado por la esterasa intracelular y se activa. El indicador activado es una molécula polar que ya no es capaz de difundirse libremente a través de la membrana celular, esencialmente atrapada dentro de las células.

Calbryte 520XL tiene baja afinidad por el ion calcio con Kd ~300 uM, que es similar al conocido Rhod 5N, pero mucho más estable que Rhod-5N. Calbryte 520XL produce una señal de fluorescencia brillante en presencia de iones de calcio en alta concentración. Tiene la misma longitud de onda de excitación y emisión que Fluo-4, por lo que la misma configuración de ensayo de Fluo-4 se puede aplicar fácilmente a los ensayos de calcio basados en Calbryte 520XL.

Calbryte 520XL es una excelente alternativa a Rhod-5N. También ofrecemos Calbryte 520XL, sal de potasio (n.º 20645), Calbryte 520XL-dextrano (n.º 20648), azida Calbryte 520XL (n.º 20643) que se pueden conjugar fácilmente con un vehículo a través de la conocida química de clic.

Nombre en ingles: Calbryte™-520XL AM

Importante: Solo para uso en investigación (RUO). Almacenamiento: Congelar a  (< -15 °C), Minimizar la exposición a la luz.

CatalogoProductoPresentación
AAT-20646Indicador Calbryte™-520XL AM10 x 50 ug

Plataforma

Microscopio de Fluorescencia

ExcitaciónFITC
EmisiónFITC
Placa RecomendadaPared negra/Fondo claro

Lector de microplacas de Fluorescencia

Excitación490
Emisión525
Cutoff515
Placa Recomendada Pared Negra / fondo claro
Especificaciones InstrumentoModo de lectura inferior / manipulación de liquidos programable

Espectro

Abrir en Advanced Spectrum Viewer

Propiedades espectrales

Excitación (nm)493
Emisión (nm)515
Rendimiento cuántico0.751
1 Calcium bound (pH 7.2)

Calculadora

Volumen de DMSO necesario para reconstituir la masa específica de Calbryte™-520XL AM a la concentración dada. Tenga en cuenta que el volumen es solo para preparar la solución madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales/fisiológicos apropiados.

uffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM109.677 µL548.384 µL1.097 mL5.484 mL10.968 mL
5 mM21.935 µL109.677 µL219.354 µL1.097 mL2.194 mL
10 mM10.968 µL54.838 µL109.677 µL548.384 µL1.097 mL

Imagen

Figura 1. Emisión de fluorescencia dependiente de Ca2+ del indicador Calbryte™-520XL (Ex/Em = 490/525 nm).

Productos Relacionados

NameExcitation (nm)Emission (nm)Quantum yield
Calbryte™ 520, potassium salt4935150.751
Calbryte™ 590, potassium salt581593
Calbryte™ 630, potassium salt607624
Calbryte™-520XL, potassium salt4935150.751
1 Calcium bound (pH 7.2)

Calbryte™ 520 AM
Calbryte™ 590 AM
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Calbryte™-520L AM
Calbryte™-520XL azide
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Calbryte™-520XL-Dextran
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Cal Green™ 1, AM [Equivalent to Calcium Green-1, AM]
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Cal-590™-Dextran Conjugate *MW 10,000*
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Cal-590™, sodium salt
Cal-590™, potassium salt
Cal-630™ AM
Cal-630™, sodium salt
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Cal-630™-Dextran Conjugate *MW 10,000*
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Cal Red™ R525/650 potassium salt
Cal Red™ R525/650 AM
Cal-520®-Dextran Conjugate *MW 3,000*
Cal-520®-Dextran Conjugate *MW 10,000*
Cal-520®-Biotin Conjugate
Cal-520®-Biocytin Conjugate
Cal-520® NHS Ester
Cal-520® maleimide
Fluo-3, AM *CAS 121714-22-5*
Fluo-3, AM *UltraPure grade* *CAS 121714-22-5*
Fluo-3, AM *Bulk package* *CAS 121714-22-5*
Fluo-3FF, AM *UltraPure grade* *Cell permeant*
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Bibliografía

Ver todas las 13 bibliografias: Citation Explorer

Calreticulin regulates TGF-&beta;1-induced epithelial mesenchymal transition through modulating Smad signaling and calcium signaling
Authors: Wu, Yanjiao and Xu, Xiaoli and Ma, Lunkun and Yi, Qian and Sun, Weichao and Tang, Liling
Journal: The International Journal of Biochemistry &amp; Cell Biology (2017)

Monosialoganglioside 1 may alleviate neurotoxicity induced by propofol combined with remifentanil in neural stem cells
Authors: Lu, Jiang and Yao, Xue-qin and Luo, Xin and Wang, Yu and Chung, Sookja Kim and Tang, He-xin and Cheung, Chi Wai and Wang, Xian-yu and Meng, Chen and Li, Qing and others, undefined
Journal: Neural Regeneration Research (2017): 945

Obtaining spontaneously beating cardiomyocyte-like cells from adipose-derived stromal vascular fractions cultured on enzyme-crosslinked gelatin hydrogels
Authors: Yang, Gang and Xiao, Zhenghua and Ren, Xiaomei and Long, Haiyan and Ma, Kunlong and Qian, Hong and Guo, Yingqiang
Journal: Scientific Reports (2017): 41781

Dexmedetomidine reduces hypoxia/reoxygenation injury by regulating mitochondrial fission in rat hippocampal neurons
Authors: Liu, Jia and Du, Qing and Zhu, He and Li, Yu and Liu, Maodong and Yu, Shoushui and Wang, Shilei
Journal: Int J Clin Exp Med (2017): 6861–6868

Di (2-ethylhexyl) phthalate-induced apoptosis in rat INS-1 cells is dependent on activation of endoplasmic reticulum stress and suppression of antioxidant protection
Authors: Sun, Xia and Lin, Yi and Huang, Qiansheng and Shi, Junpeng and Qiu, Ling and Kang, Mei and Chen, Yajie and Fang, Chao and Ye, Ting and Dong, Sijun
Journal: Journal of cellular and molecular medicine (2015): 581–594

The effect of mitochondrial calcium uniporter on mitochondrial fission in hippocampus cells ischemia/reperfusion injury
Authors: Zhao, Lantao and Li, Shuhong and Wang, Shilei and Yu, Ning and Liu, Jia
Journal: Biochemical and biophysical research communications (2015): 537–542

Role of mitochondrial calcium uniporter in regulating mitochondrial fission in the cerebral cortexes of living rats
Authors: Liang, Nan and Wang, Peng and Wang, Shilei and Li, Shuhong and Li, Yu and Wang, Jinying and Wang, Min
Journal: Journal of Neural Transmission (2014): 593–600

Propofol and remifentanil at moderate and high concentrations affect proliferation and differentiation of neural stem/progenitor cells
Authors: Li, Qing and Lu, Jiang and Wang, Xianyu and others, undefined
Journal: Neural regeneration research (2014): 2002

Fungus induces the release of IL-8 in human corneal epithelial cells, via Dectin-1-mediated protein kinase C pathways.
Authors: Peng, Xu-Dong and Zhao, Gui-Qiu and Lin, Jing and Jiang, Nan and Xu, Qiang and Zhu, Cheng-Cheng and Qu, Jain-Qiu and Cong, Lin and Li, Hui
Journal: International journal of ophthalmology (2014): 441–447

Increased expression of cell adhesion molecule 1 by mast cells as a cause of enhanced nerve–mast cell interaction in a hapten-induced mouse model of atopic dermatitis
Authors: Hagiyama, M and Inoue, T and Furuno, T and Iino, T and Itami, S and Nakanishi, M and Asada, H and Hosokawa, Y and Ito, A
Journal: British Journal of Dermatology (2013): 771–778

Referencias

Ver todas las 53 referencias: Citation Explorer

A flow cytometric comparison of Indo-1 to fluo-3 and Fura Red excited with low power lasers for detecting Ca(2+) flux
Authors: Bailey S, Macardle PJ.
Journal: J Immunol Methods (2006): 220

Functional fluo-3/AM assay on P-glycoprotein transport activity in L1210/VCR cells by confocal microscopy
Authors: Orlicky J, Sulova Z, Dovinova I, Fiala R, Zahradnikova A, Jr., Breier A.
Journal: Gen Physiol Biophys (2004): 357

Comparison of human recombinant adenosine A2B receptor function assessed by Fluo-3-AM fluorometry and microphysiometry
Authors: Patel H, Porter RH, Palmer AM, Croucher MJ.
Journal: Br J Pharmacol (2003): 671

Measurement of the dissociation constant of Fluo-3 for Ca2+ in isolated rabbit cardiomyocytes using Ca2+ wave characteristics
Authors: Loughrey CM, MacEachern KE, Cooper J, Smith GL.
Journal: Cell Calcium (2003): 1

A sensitive method for the detection of foot and mouth disease virus by in situ hybridisation using biotin-labelled oligodeoxynucleotides and tyramide signal amplification
Authors: Zhang Z, Kitching P.
Journal: J Virol Methods (2000): 187

Kinetics of onset of mouse sperm acrosome reaction induced by solubilized zona pellucida: fluorimetric determination of loss of pH gradient between acrosomal lumen and medium monitored by dapoxyl (2-aminoethyl) sulfonamide and of intracellular Ca(2+) chang
Authors: Rockwell PL, Storey BT.
Journal: Mol Reprod Dev (2000): 335

MRP2, a human conjugate export pump, is present and transports fluo 3 into apical vacuoles of Hep G2 cells
Authors: Cantz T, Nies AT, Brom M, Hofmann AF, Keppler D.
Journal: Am J Physiol Gastrointest Liver Physiol (2000): G522

Use of co-loaded Fluo-3 and Fura Red fluorescent indicators for studying the cytosolic Ca(2+)concentrations distribution in living plant tissue
Authors: Walczysko P, Wagner E, Albrechtova JT.
Journal: Cell Calcium (2000): 23

[Ca2+]i following extrasystoles in guinea-pig trabeculae microinjected with fluo-3 – a comparison with frog skeletal muscle fibres
Authors: Wohlfart B., undefined
Journal: Acta Physiol Scand (2000): 1

Determination of the intracellular dissociation constant, K(D), of the fluo-3. Ca(2+) complex in mouse sperm for use in estimating intracellular Ca(2+) concentrations
Authors: Rockwell PL, Storey BT.
Journal: Mol Reprod Dev (1999): 418

Aplication Notes

A Comparison of Fluorescent Red Calcium Indicators for Detecting Intracellular Calcium Mobilization in CHO Cells
A Meta-Analysis of Common Calcium Indicators
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets

FAQ

Are there any calcium indicators that don’t require probenecid (PBC)?
Are there upgraded trypan blue derivatives for cell viability testing?
Can I intracellularly measure mitochondria calcium flux and changes in mitochondria membrane potential at the same time?
Do you offer any products for measuring intracellular calcium concentration or movement by flow cytometry?
How do I make an AM ester stock solution?

AssayWise

Multicolor Intracellular Calcium Detection Probes
Cal-520™ Calcium Indicators
Cal-590™ Calcium Indicators
Cal-630™ Calcium Indicators
Fluo-8® Calcium Indicators