Indicador Calbryte™ 520 Sal de potasio

El indicador Calbryte™ 520 sal de potasio es una nueva generación de indicadores fluorescentes para mediciones de calcio. Su mejorada relación señal/ruido y mejores propiedades de retención intracelular hacen de Calbryte™ 520 el indicador de calcio más sólido.


Indicador Calbryte™ 520 Sal de potasio

La medición del calcio es fundamental para numerosas investigaciones biológicas. Las sondas fluorescentes que muestran respuestas espectrales al unirse al calcio han permitido a los cientificos investigar los cambios en las concentraciones de calcio libre celular mediante microscopía de fluorescencia, citometría de flujo, espectroscopía de fluorescencia y lectores de microplacas de fluorescencia.

Seguido por la introducción de Fluo-3 en 1989, Fluo-4, Fluo-8 y Cal-520 se desarrollaron más tarde con una mejor relación señal/fondo. Sin embargo, todavía hay algunos problemas graves con Fluo-4. Por ejemplo, en cuanto a Fluo-3, en la mayoría de los ensayos de calcio intracelular con Fluo-4, se requiere probenecid para evitar que el Fluo-4 cargado en las células se escape de las células. El uso de probenecid con ensayos de calcio basados en Fluo-4 compromete los resultados del ensayo ya que está bien documentado que el probenecid tiene una variedad de efectos celulares complicados.

Calbryte™ 520 es una nueva generación de indicadores fluorescentes para mediciones de calcio, su relación señal/ruido muy mejorada y mejores propiedades de retención intracelular hacen de Calbryte™ 520 el indicador de calcio más sólido. Tiene la misma longitud de onda de excitación y emisión que Fluo-4, por lo que la misma configuración de ensayo de Fluo-4 se puede aplicar fácilmente a los ensayos de calcio basados en Calbryte™ 520.

AAT-20656Calbryte™ 520, potassium salt2 x 50 ug
AAT-20657Calbryte™ 520, potassium salt1 mg
AAT-20658Calbryte™ 520, potassium salt10 x 50 ug

Importante: Solo para uso en investigación (RUO). Almacenamiento: Congelar a  (< -15 °C), Minimizar la exposición a la luz.


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Propiedades espectrales

Excitación (nm)493
Emisión (nm)515
Rendimiento cuántico0.751
1 Calcium bound (pH 7.2)


Preparación de la solución de stock común
Volumen de agua necesario para reconstituir la masa específica de Calbryte™ 520, sal de potasio a la concentración dada. Tenga en cuenta que el volumen es solo para preparar la solución madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales/fisiológicos apropiados.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM110.009 µL550.043 µL1.1 mL5.5 mL11.001 mL
5 mM22.002 µL110.009 µL220.017 µL1.1 mL2.2 mL
10 mM11.001 µL55.004 µL110.009 µL550.043 µL1.1 mL


Figura 1. Espectros de emisión de fluorescencia de Calbryte™ 520 en una solución que contiene de 0 a 39 µM de Ca2+ libre.

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