iFluor® 750 succinimidyl ester

iFluor 750

La familia de colorantes iFluor 750 con características espectrales especificas los convierten en una alternativa superior a Cy7® y Alexa Fluor® 750 de GE Healthcare e Invitrogen respectivamente.

Descripción

Los tintes iFluor® de AAT Bioquest están desarrollados para marcar proteínas, en particular anticuerpos. Estos tintes están optimizados para tener un efecto mínimo de extinción de la fluorescencia en proteínas y ácidos nucleicos. Nuestros tintes iFluor® 750 tienen un máximo de excitación y emisión de fluorescencia de ~757 nm y ~779 nm, respectivamente, con buena fotoestabilidad.

Nuestros estudios comparables internos indicaron que nuestros tintes iFluor® 750 son significativamente más brillantes que los correspondientes Cy7® y Alexa Fluor® 750. Estas características espectrales los convierten en una alternativa superior a Cy7® y Alexa Fluor® 750 (Cy7® y Alexa Fluor® son marcas comerciales de GE Healthcare e Invitrogen).

Los conjugados iFluor® 750 se han utilizado ampliamente en aplicaciones de imágenes de animales por fluorescencia. El tinte iFluor® 750 SE es razonablemente estable y muestra buena reactividad y selectividad con las proteínas.

CatalogoProductoPresentación
AAT-1037iFluor® 750 succinimidyl ester1mg
AAT-71037iFluor® 750 succinimidyl ester100 ug
AAT-71515iFluor® 750 succinimidyl ester5mg
AAT-71565iFluor® 750 succinimidyl ester10mg

Importante: Solo para uso en investigación (RUO). Almacenamiento: Congelación (< -15 °C). Minimizar la exposición a la luz.

Propiedades fisicas

Peso Molecular 1416.83
DisolventeDMSO

Espectro

Abrir en Advanced Spectrum Viewer

Propiedades espectrales

Factor de corrección (260 nm)0.044
Factor de corrección (280 nm)0.039
Factor de corrección (565 nm)0.0250
Factor de corrección (650 nm)0.1413
Coeficiente de extinción (cm -1 M -1)2750001
Excitación (nm)757
Emisión (nm)779
Rendimiento cuántico0.121
1 Buffer acuoso (pH 7,2)

Calculadora

Preparación de la solución de stock común

Volumen de DMSO necesario para reconstituir la masa específica de succinimidil éster iFluor® 750 a la concentración dada. Tenga en cuenta que el volumen es solo para preparar la solución madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales/fisiológicos apropiados.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM70.58 µL352.9 µL705.801 µL3.529 mL7.058 mL
5 mM14.116 µL70.58 µL141.16 µL705.801 µL1.412 mL
10 mM7.058 µL35.29 µL70.58 µL352.9 µL705.801 µL

Imagen

Figura 1. Las células HL-60 se incubaron con (Rojo, +) o sin (Verde, -) HLA-ABC antihumano (W6/32 mAb), seguido de conjugado de IgG anti-ratón de cabra iFluor® 750. La señal de fluorescencia se controló utilizando un citómetro de flujo ACEA NovoCyte en el canal APC-Cy7.

Productos Similares

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
iFluor® 350 succinimidyl ester3454502000010.9510.830.23
iFluor® 405 succinimidyl ester4034273700010.9110.480.77
iFluor® 488 succinimidyl ester4915167500010.910.210.11
iFluor® 514 succinimidyl ester5115277500010.8310.2650.116
iFluor® 532 succinimidyl ester5375609000010.6810.260.16
iFluor® 555 succinimidyl ester55757010000010.6410.230.14
iFluor® 633 succinimidyl ester64065425000010.2910.0620.044
iFluor® 647 succinimidyl ester65667025000010.2510.030.03
iFluor® 660 succinimidyl ester66367825000010.2610.070.08
iFluor® 680 succinimidyl ester68470122000010.2310.0970.094
iFluor® 700 succinimidyl ester69071322000010.2310.090.04
iFluor® 750 succinimidyl ester75777927500010.1210.0440.039
iFluor® 610 succinimidyl ester61062811000010.8510.320.49
iFluor® 710 succinimidyl ester71773919000010.6010.120.07
iFluor® 790 succinimidyl ester78781225000010.1310.10.09
iFluor® 800 succinimidyl ester80182025000010.1110.030.08
iFluor® 810 succinimidyl ester81182225000010.0510.090.15
iFluor® 820 succinimidyl ester82285025000010.110.16
iFluor® 860 succinimidyl ester85387825000010.10.14
iFluor® 546 succinimidyl ester54155710000010.6710.250.15
iFluor® 568 succinimidyl ester56858710000010.5710.340.15
iFluor® 430 succinimidyl ester4334984000010.7810.680.3
iFluor® 450 succinimidyl ester4515024000010.8210.450.27
iFluor® 840 succinimidyl ester83687920000010.20.09
iFluor® 560 succinimidyl ester56057112000010.5710.04820.069
iFluor® 670 succinimidyl ester67168220000010.5510.030.033
iFluor® 460 succinimidyl ester468493800001~0.810.980.46
iFluor® 440 succinimidyl ester4344804000010.6710.3520.229
iFluor® 665 succinimidyl ester667692110,00010.2210.120.09
iFluor® 690 succinimidyl ester68570422000010.3010.090.06
iFluor® A7 SE
iFluor® 350 maleimide
iFluor® 488 maleimide
iFluor® 555 maleimide
iFluor® 647 maleimide
iFluor® 680 maleimide
iFluor® 700 maleimide
iFluor® 750 maleimide
iFluor® 350 amine
iFluor® 405 amine
iFluor® 488 amine
iFluor® 555 amine
iFluor® 647 amine
iFluor® 660 amine
iFluor® 680 amine
iFluor® 700 amine
iFluor® 710 amine
iFluor® 750 amine
iFluor® 350 hydrazide
iFluor® 488 hydrazide
iFluor® 555 hydrazide
iFluor® 647 hydrazide
iFluor® 680 hydrazide
iFluor® 700 hydrazide
iFluor® 750 hydrazide
iFluor® 647 alkyne
iFluor® 647 azide
iFluor® 790 acid
iFluor® 790 amine
iFluor® 790 hydrazide
iFluor® 790 maleimide

Bibliografiía

Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification
Authors: Gao, D and Liu, N and Li, Y and Zhang, Y and Liu, G and others, undefined
Journal: Metabolomics (Los Angel) (2017): 2153–0769

Suramin inhibits cullin-RING E3 ubiquitin ligases
Authors: Wu, Kenneth and Chong, Robert A and Yu, Qing and Bai, Jin and Spratt, Donald E and Ching, Kevin and Lee, Chan and Miao, Haibin and Tappin, Inger and Hurwitz, Jerard and others, undefined
Journal: Proceedings of the National Academy of Sciences (2016): E2011–E2018

Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function
Authors: Piccard, Helene and Berghmans, Nele and Korpos, Eva and Dillen, Chris and Aelst, Ilse Van and Li, S and ra , undefined and Martens, Erik and Liekens, S and ra , undefined and Noppen, Sam and Damme, Jo Van and others, undefined
Journal: International Journal of Cancer (2012): E425–E436

In Vivo Visualization of Collagen Transdermal Absorption by a Combined Second-harmonic Generation and Two-photon Excited Fluorescence Method
Authors: Ma, Shuhua and He, Gaiying and Yang, Weifeng and Wang, Yi and others,
Journal: (2021)

Referencias

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Visualizing dengue virus through Alexa Fluor labeling
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Journal: J Vis Exp. (2011)

Fluorescent “Turn-on” system utilizing a quencher-conjugated peptide for specific protein labeling of living cells
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Neuroanatomical basis of clinical joint application of “Jinggu” (BL 64, a source-acupoint) and “Dazhong” (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594
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Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers
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Journal: J Microbiol Methods (2011): 119

Alexa Fluor 546-ArIB[V11L;V16A] is a potent ligand for selectively labeling alpha 7 nicotinic acetylcholine receptors
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Novel Alexa Fluor-488 labeled antagonist of the A(2A) adenosine receptor: Application to a fluorescence polarization-based receptor binding assay
Authors: Kecskes M, Kumar TS, Yoo L, Gao ZG, Jacobson KA.
Journal: Biochem Pharmacol (2010): 506

Application Notes

iFluor® Dye Selection Guide (Guia de selección)
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)

FAQ

What are the spectral properties of iFluor dyes?
Are any of the cyanine dyes infrared?
Are coumarin dyes pH sensitive?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?

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