La nueva familia de colorantes iFluor 680 son una excelente alternativa a los colorantes de etiquetado Cy5.5® y Alexa Fluor® 680.
Descripción
Los tintes iFluor® de AAT Bioquest están optimizados para marcar proteínas, en particular anticuerpos. Estos tintes son brillantes, fotoestables y tienen un enfriamiento mínimo de las proteínas. Pueden excitarse bien con las principales líneas láser de los instrumentos de fluorescencia (ejem. 350, 405, 488, 555 y 633 nm).
Los tintes iFluor® 680 tienen un máximo de excitación y emisión de fluorescencia de ~684 nm y ~701 nm respectivamente. Estas características espectrales los convierten en una excelente alternativa a los colorantes de etiquetado Cy5.5® y Alexa Fluor® 680 (Cy5.5® y Alexa Fluor® son marcas comerciales de GE Health Care e Invitrogen).
iFluor® 680 SE es razonablemente estable y muestra buena reactividad y selectividad con los grupos amino de proteínas.
Catalogo | Producto | Presentación |
---|---|---|
AAT-1035 | iFluor® 680 succinimidyl ester | 1mg |
AAT-71035 | iFluor® 680 succinimidyl ester | 100 ug |
AAT-71513 | iFluor® 680 succinimidyl ester | 5mg |
AAT-71563 | iFluor® 680 succinimidyl ester | 10mg |
Importante: Solo para uso en investigación (RUO). Almacenamiento: Congelación (< -15 °C). Minimizar la exposición a la luz.
Propiedades fisicas
Peso Molecular | 957.17 |
Disolvente | DMSO |
Espectro
Abrir en Advanced Spectrum Viewer
Propiedades espectrales
Factor de corrección (260 nm) | 0.097 |
Factor de corrección (280 nm) | 0.094 |
Coeficiente de extinción (cm -1 M -1) | 2200001 |
Excitación (nm) | 684 |
Emisión (nm) | 701 |
Rendimiento cuántico | 0.231 |
Calculadora
Preparación de la solución de stock común
Volumen de DMSO necesario para reconstituir la masa específica de succinimidil éster iFluor® 680 a la concentración dada. Tenga en cuenta que el volumen es solo para preparar la solución madre. Consulte el protocolo experimental de la muestra para conocer los buffers experimentales/fisiológicos apropiados.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 104.475 µL | 522.373 µL | 1.045 mL | 5.224 mL | 10.447 mL |
5 mM | 20.895 µL | 104.475 µL | 208.949 µL | 1.045 mL | 2.089 mL |
10 mM | 10.447 µL | 52.237 µL | 104.475 µL | 522.373 µL | 1.045 mL |
Imagenes
Figura 1. Las células HeLa se tiñeron con antitubulina de ratón seguido de iFluorTM 680 de cabra anti-IgG de ratón (H+L); y los núcleos se tiñeron con Nuclear GreenTM DCS1 (Cat# 17550).
Figura 2. Análisis de citometría de flujo de PBMC teñidas con iFluor® 680 conjugado anti-CD4 *SK3* humano. La señal de fluorescencia se controló utilizando un citómetro de flujo Aurora en el canal R4-A específico de iFluor® 680.
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Bibliografiía
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Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function
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Journal: International Journal of Cancer (2012): E425–E436
Referencias
Verw todas las 49 referencias: Citation Explorer
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Journal: Mol Biosyst (2011): 1766
Visualizing dengue virus through Alexa Fluor labeling
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Neuroanatomical basis of clinical joint application of “Jinggu” (BL 64, a source-acupoint) and “Dazhong” (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594
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Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers
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Application Notes
iFluor® Dye Selection Guide
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
FAQ
What are the spectral properties of iFluor dyes?
Are any of the cyanine dyes infrared?
Are coumarin dyes pH sensitive?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
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