iFluor® 647 SE es razonablemente estable y muestra buena reactividad y selectividad con los grupos amino de proteínas.
Descripción
Los tintes iFluor® de AAT Bioquest están optimizados para marcar proteínas, en particular anticuerpos. Estos tintes son brillantes, fotoestables y tienen un enfriamiento mínimo de las proteínas. Pueden excitarse bien con las principales líneas láser de los instrumentos de fluorescencia (p. ej., 350, 405, 488, 555 y 633 nm). Los colorantes iFluor® 700 tienen un máximo de excitación y emisión de fluorescencia de ~690 nm y ~713 nm respectivamente. Estas características espectrales los convierten en una excelente alternativa al colorante de etiquetado Alexa Fluor® 700 (Alexa Fluor® es la marca comercial de Invitrogen). iFluor® 700 SE es razonablemente estable y muestra buena reactividad y selectividad con los grupos amino de proteínas.
| Catalogo | Producto | Presentación |
|---|---|---|
| AAT-1031 | iFluor® 647 succinimidyl ester | 1mg |
| AAT-71031 | iFluor® 647 succinimidyl ester | 100 ug |
| AAT-71510 | iFluor® 647 succinimidyl ester | 5mg |
| AAT-71560 | iFluor® 647 succinimidyl ester | 10mg |
Importante: Solo para uso en investigación (RUO). Almacenamiento: Congelación (< -15 °C). Minimizar la exposición a la luz.
Propiedades fisicas
| Peso Molecular | 1274.66 |
| Disolvente | DMSO |
Espectro
Abrir en Advanced Spectrum Viewer
Propiedades espectrales
| Factor de corrección (260 nm) | 0.03 |
| Factor de corrección (280 nm) | 0.03 |
| Factor de corrección (656 nm) | 0.0793 |
| Coeficiente de extinción (cm -1 M -1) | 2500001 |
| Excitación (nm) | 656 |
| Emisión (nm) | 670 |
| Rendimiento cuántico | 0.251 |
Calculadora
Preparación de la solución de stock común
Volumen de DMSO necesario para reconstituir la masa específica de succinimidil éster iFluor® 700 a la concentración dada. Tenga en cuenta que el volumen es solo para preparar la solución madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales/fisiológicos apropiados.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 78.452 µL | 392.261 µL | 784.523 µL | 3.923 mL | 7.845 mL |
| 5 mM | 15.69 µL | 78.452 µL | 156.905 µL | 784.523 µL | 1.569 mL |
| 10 mM | 7.845 µL | 39.226 µL | 78.452 µL | 392.261 µL | 784.523 µL |
Imagenes
Figura 1. Las células HeLa se tiñeron con antitubulina de ratón seguido de iFluorTM 647 de cabra anti-ratón IgG (H+L) (rojo); y los núcleos se tiñeron con DAPI (azul).
Figura 2. Las células HeLa se incubaron con antitubulina de ratón seguida de conjugado de IgG anti-ratón de cabra iFluorTM 647 de AAT (rojo, izquierda) o IgG anti-ratón de cabra Alexa Fluor® 647 (rojo, derecha), respectivamente. Los núcleos celulares se tiñeron con Hoechst 33342 (Blue, Cat#17530).
Figura 3 Las células HeLa se incubaron con antitubulina de ratón y anti-IgG de ratón de cabra con biotina, seguidas de conjugado iFluor® 647-estreptavidina de AAT (rojo, izquierda) o estreptavidina conjugada con Alexa Fluor® 647 (rojo, derecha), respectivamente.
Figura 4. Hibridación fluorescente in situ de sondas de telómero marcadas con Cy5 e iFlour® 647-dUTP en celulas HeLa en Metafase.
Productos Similares
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| iFluor® 350 succinimidyl ester | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
| iFluor® 405 succinimidyl ester | 403 | 427 | 370001 | 0.911 | 0.48 | 0.77 |
| iFluor® 514 succinimidyl ester | 511 | 527 | 750001 | 0.831 | 0.265 | 0.116 |
| iFluor® 532 succinimidyl ester | 537 | 560 | 900001 | 0.681 | 0.26 | 0.16 |
| iFluor® 555 succinimidyl ester | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
| iFluor® 594 succinimidyl ester | 588 | 604 | 1800001 | 0.531 | 0.05 | 0.04 |
| iFluor® 633 succinimidyl ester | 640 | 654 | 2500001 | 0.291 | 0.062 | 0.044 |
| iFluor® 660 succinimidyl ester | 663 | 678 | 2500001 | 0.261 | 0.07 | 0.08 |
Bibliografía
Ver todas las 36 bibliografías: Citation Explorer
Inhibition of MYC suppresses programmed cell death ligand-1 expression and enhances immunotherapy in triple-negative breast cancer
Authors: Li, Xintong and Tang, Lin and Chen, Qin and Cheng, Xumin and Liu, Yiqiu and Wang, Cenzhu and Zhu, Chengjun and Xu, Kun and Gao, Fangyan and Huang, Jinyi and others,
Journal: Chinese Medical Journal (2022): 10–1097
Immune-regulating bimetallic metal-organic framework nanoparticles designed for cancer immunotherapy
Authors: Dai, Zan and Wang, Qiaoyun and Tang, Jie and Wu, Min and Li, Haoze and Yang, Yannan and Zhen, Xu and Yu, Chengzhong
Journal: Biomaterials (2022): 121261
Preparation and identification of monoclonal antibodies against porcine CD103
Authors: Zhang, Tao and Yu, Haoyuan and Aryal, Manita and Yang, Jing and Li, Maolin and Li, Shuxian and Zhang, Na and Shi, Han and Li, Baoyu and Liu, Guangliang and others,
Journal: Applied Microbiology and Biotechnology (2022): 1–11
Site-specific labeling and functional efficiencies of human fibroblast growth Factor-1 with a range of fluorescent Dyes in the flexible N-Terminal region and a rigid $\beta$-turn region
Authors: Mohale, Mamello and Gundampati, Ravi Kumar and Kumar, Thallapuranam Krishnaswamy Suresh and Heyes, Colin D
Journal: Analytical biochemistry (2022): 114524
A nano-innate immune system activator for cancer therapy in a 4T1 tumor-bearing mouse model
Authors: Liu, Xiang-Yu and Zhu, Mao-Hua and Wang, Xiao-Yu and Dong, Xiao and Liu, Hai-Jun and Li, Rui-Yang and Jia, Shi-Chong and Lu, Qin and Zhao, Mei and Sun, Peng and others,
Journal: Journal of nanobiotechnology (2022): 1–15
Poly ADP-ribosylation of SET8 leads to aberrant H4K20 methylation in mammalian nuclear genome
Authors: Est{\`e}ve, Pierre-Olivier and Sen, Sagnik and Vishnu, Udayakumar S and Ruse, Cristian and Chin, Hang Gyeong and Pradhan, Sriharsa
Journal: Communications biology (2022): 1–18
The PARP1 Inhibitor Niraparib Represses DNA Damage Repair and Synergizes with Temozolomide for Antimyeloma Effects
Authors: Shen, Hong-Yuan and Tang, Hai-Long and Zheng, Yan-Hua and Feng, Juan and Dong, Bao-Xia and Chen, Xie-Qun
Journal: Journal of Oncology (2022)
A Substance P (SP)/Neurokinin-1 Receptor Axis Promotes Perineural Invasion of Pancreatic Cancer and Is Affected by lncRNA LOC389641
Authors: Ji, Tengfei and Ma, Keqiang and Wu, Hongsheng and Cao, Tiansheng
Journal: Journal of Immunology Research (2022)
RNA-Editing Enzyme ADAR1 p150 Isoform Is Critical for Germinal Center B Cell Response
Authors: Li, Yuxing and Ruan, Gui-Xin and Chen, Wenjing and Huang, Hengjun and Zhang, Rui and Wang, Jing and Li, Yan and Xu, Shengli and Ou, Xijun
Journal: The Journal of Immunology (2022): 1071–1082
Increased maternofoetal transfer of antibodies in a murine model of systemic lupus erythematosus, but no immune activation and neuroimmune sequelae in offspring
Authors: Fonager, Sofie Vestergaard and Winther, Gudrun and Wittenborn, Thomas Rea and Jensen, Lisbeth and Fahlquist-Hagert, Cecilia and Hansen, Lisbeth Ahm and F{\”u}chtbauer, Ernst-Martin and Romero-Ramos, Marina and Degn, S{\o}ren Egedal
Journal: Journal of Neuroimmunology (2022)
Referencias
Ver todas las 49 referencias: Citation Explorer
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Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers
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Alexa Fluor 546-ArIB[V11L;V16A] is a potent ligand for selectively labeling alpha 7 nicotinic acetylcholine receptors
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Novel Alexa Fluor-488 labeled antagonist of the A(2A) adenosine receptor: Application to a fluorescence polarization-based receptor binding assay
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Application Notes (en Ingles)
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)
iFluor® Dye Selection Guide
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)