iFluor® 647 succinimidyl ester

iFluor® 647 SE es razonablemente estable y muestra buena reactividad y selectividad con los grupos amino de proteínas.


Los tintes iFluor® de AAT Bioquest están optimizados para marcar proteínas, en particular anticuerpos. Estos tintes son brillantes, fotoestables y tienen un enfriamiento mínimo de las proteínas. Pueden excitarse bien con las principales líneas láser de los instrumentos de fluorescencia (p. ej., 350, 405, 488, 555 y 633 nm). Los colorantes iFluor® 700 tienen un máximo de excitación y emisión de fluorescencia de ~690 nm y ~713 nm respectivamente. Estas características espectrales los convierten en una excelente alternativa al colorante de etiquetado Alexa Fluor® 700 (Alexa Fluor® es la marca comercial de Invitrogen). iFluor® 700 SE es razonablemente estable y muestra buena reactividad y selectividad con los grupos amino de proteínas.

AAT-1031iFluor® 647 succinimidyl ester1mg
AAT-71031iFluor® 647 succinimidyl ester100 ug
AAT-71510iFluor® 647 succinimidyl ester5mg
AAT-71560iFluor® 647 succinimidyl ester10mg

Importante: Solo para uso en investigación (RUO). Almacenamiento: Congelación (< -15 °C). Minimizar la exposición a la luz.

Propiedades fisicas

Peso Molecular 1274.66


Abrir en Advanced Spectrum Viewer

Propiedades espectrales

Factor de corrección (260 nm)0.03
Factor de corrección (280 nm)0.03
Factor de corrección (656 nm)0.0793
Coeficiente de extinción (cm -1 M -1)2500001
Excitación (nm)656
Emisión (nm)670
Rendimiento cuántico0.251
1 Buffer acuoso (pH 7,2)


Preparación de la solución de stock común

Volumen de DMSO necesario para reconstituir la masa específica de succinimidil éster iFluor® 700 a la concentración dada. Tenga en cuenta que el volumen es solo para preparar la solución madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales/fisiológicos apropiados.

 0.1 mg0.5 mg1 mg5 mg10 mg
1 mM78.452 µL392.261 µL784.523 µL3.923 mL7.845 mL
5 mM15.69 µL78.452 µL156.905 µL784.523 µL1.569 mL
10 mM7.845 µL39.226 µL78.452 µL392.261 µL784.523 µL


Fig. 1

Figura 1.  Las células HeLa se tiñeron con antitubulina de ratón seguido de iFluorTM 647 de cabra anti-ratón IgG (H+L) (rojo); y los núcleos se tiñeron con DAPI (azul).

Fig. 2

Figura 2Las células HeLa se incubaron con antitubulina de ratón seguida de conjugado de IgG anti-ratón de cabra iFluorTM 647 de AAT (rojo, izquierda) o IgG anti-ratón de cabra Alexa Fluor® 647 (rojo, derecha), respectivamente. Los núcleos celulares se tiñeron con Hoechst 33342 (Blue, Cat#17530).

Fig. 3

Figura 3  Las células HeLa se incubaron con antitubulina de ratón y anti-IgG de ratón de cabra con biotina, seguidas de conjugado iFluor® 647-estreptavidina de AAT (rojo, izquierda) o estreptavidina conjugada con Alexa Fluor® 647 (rojo, derecha), respectivamente.

Fig 4.

Figura 4.  Hibridación fluorescente in situ de sondas de telómero marcadas con Cy5 e iFlour® 647-dUTP en celulas HeLa en Metafase.

Productos Similares

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
iFluor® 350 succinimidyl ester3454502000010.9510.830.23
iFluor® 405 succinimidyl ester4034273700010.9110.480.77
iFluor® 514 succinimidyl ester5115277500010.8310.2650.116
iFluor® 532 succinimidyl ester5375609000010.6810.260.16
iFluor® 555 succinimidyl ester55757010000010.6410.230.14
iFluor® 594 succinimidyl ester58860418000010.5310.050.04
iFluor® 633 succinimidyl ester64065425000010.2910.0620.044
iFluor® 660 succinimidyl ester66367825000010.2610.070.08


Ver todas las 36 bibliografías: Citation Explorer

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A nano-innate immune system activator for cancer therapy in a 4T1 tumor-bearing mouse model
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The PARP1 Inhibitor Niraparib Represses DNA Damage Repair and Synergizes with Temozolomide for Antimyeloma Effects
Authors: Shen, Hong-Yuan and Tang, Hai-Long and Zheng, Yan-Hua and Feng, Juan and Dong, Bao-Xia and Chen, Xie-Qun
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A Substance P (SP)/Neurokinin-1 Receptor Axis Promotes Perineural Invasion of Pancreatic Cancer and Is Affected by lncRNA LOC389641
Authors: Ji, Tengfei and Ma, Keqiang and Wu, Hongsheng and Cao, Tiansheng
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RNA-Editing Enzyme ADAR1 p150 Isoform Is Critical for Germinal Center B Cell Response
Authors: Li, Yuxing and Ruan, Gui-Xin and Chen, Wenjing and Huang, Hengjun and Zhang, Rui and Wang, Jing and Li, Yan and Xu, Shengli and Ou, Xijun
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Increased maternofoetal transfer of antibodies in a murine model of systemic lupus erythematosus, but no immune activation and neuroimmune sequelae in offspring
Authors: Fonager, Sofie Vestergaard and Winther, Gudrun and Wittenborn, Thomas Rea and Jensen, Lisbeth and Fahlquist-Hagert, Cecilia and Hansen, Lisbeth Ahm and F{\”u}chtbauer, Ernst-Martin and Romero-Ramos, Marina and Degn, S{\o}ren Egedal
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Application Notes (en Ingles)

FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)
iFluor® Dye Selection Guide
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)