iFluor® 440 succinimidyl ester

Los conjugados de colorantes iFluor® 440 son significativamente más brillantes que los correspondientes bioconjugados de DEAC u otros colorantes espectralmente similares (como SpectrumAqua).


Los tintes iFluor® de AAT Bioquest están optimizados para marcar proteínas, en particular anticuerpos. Estos tintes son brillantes, fotoestables y tienen un enfriamiento mínimo de las proteínas. Pueden excitarse bien con las principales líneas láser de los instrumentos de fluorescencia (p. ej., 350, 405, 488, 555 y 633 nm).

Los tintes iFluor® 440 están diseñados para ser un reemplazo superior del tinte DEAC que tiene una solubilidad en agua extremadamente baja. iFluor® 440 SE es razonablemente estable y muestra buena reactividad y selectividad con los grupos amino de proteínas. En las mismas condiciones, los conjugados de colorantes iFluor® 440 son significativamente más brillantes que los correspondientes bioconjugados de DEAC u otros colorantes espectralmente similares (como SpectrumAqua), lo que hace que los conjugados iFluor® 440 sean mucho más sensibles.

AAT-1041iFluor® 440 succinimidyl ester1mg
AAT-71041iFluor® 440 succinimidyl ester100 ug
AAT-71519iFluor® 440 succinimidyl ester5mg
AAT-71569iFluor® 440 succinimidyl ester10mg

Importante: Solo para uso en investigación (RUO). Almacenamiento: Congelación (< -15 °C). Minimizar la exposición a la luz.

Propiedades fisicas

Peso Molecular 692.83


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Propiedades espectrales

Absorbancia (nm)430
Factor de corrección (260 nm)0.352
Factor de corrección (280 nm)0.229
Coeficiente de extinción (cm -1 M -1)400001
Excitación (nm)434
Emisión (nm)480
Rendimiento cuántico0.671
1 Buffer acuoso (pH 7,2)


Preparación de la solución de stock común

Volumen de DMSO necesario para reconstituir la masa específica de succinimidil éster iFluor® 440 a la concentración dada. Tenga en cuenta que el volumen es solo para preparar la solución madre. Consulte el protocolo experimental de la muestra para conocer los buffers experimentales/fisiológicos apropiados.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM144.336 µL721.678 µL1.443 mL7.217 mL14.434 mL
5 mM28.867 µL144.336 µL288.671 µL1.443 mL2.887 mL
10 mM14.434 µL72.168 µL144.336 µL721.678 µL1.443 mL


Figura 1. Las células HeLa se incubaron con antitubulina de ratón seguida de conjugado de IgG anti-ratón de cabra iFluorTM 430 de AAT (izquierda) o IgG anti-ratón de cabra conjugado con Alexa Fluor® 430 (derecha), respectivamente.

Productos Similares

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
iFluor® 350 succinimidyl ester3454502000010.9510.830.23
iFluor® 488 succinimidyl ester4915167500010.910.210.11
iFluor® 514 succinimidyl ester5115277500010.8310.2650.116
iFluor® 532 succinimidyl ester5375609000010.6810.260.16
iFluor® 555 succinimidyl ester55757010000010.6410.230.14
iFluor® 594 succinimidyl ester58860418000010.5310.050.04
iFluor® 633 succinimidyl ester64065425000010.2910.0620.044
iFluor® 647 succinimidyl ester65667025000010.2510.030.03
iFluor® 660 succinimidyl ester66367825000010.2610.070.08
iFluor® A7 SE
iFluor® 350 maleimide
iFluor® 488 maleimide
iFluor® 555 maleimide
iFluor® 647 maleimide
iFluor® 680 maleimide
iFluor® 700 maleimide
iFluor® 750 maleimide
iFluor® 350 amine
iFluor® 488 amine
iFluor® 555 amine
iFluor® 647 amine
iFluor® 660 amine
iFluor® 680 amine
iFluor® 700 amine
iFluor® 710 amine
iFluor® 750 amine
iFluor® 350 hydrazide
iFluor® 488 hydrazide
iFluor® 555 hydrazide
iFluor® 647 hydrazide
iFluor® 680 hydrazide
iFluor® 700 hydrazide


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Application Notes

iFluor® Dye Selection Guide
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)


Are any of the cyanine dyes infrared?
Are coumarin dyes pH sensitive?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?


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