DCFH-DA [diacetato de 2′,7′-diclorodihidrofluoresceína] *CAS 4091-99-0*


DCFH-DA [diacetato de 2′,7′-diclorodihidrofluoresceína. Esta sonda es ampliamente utilizada para monitorear procesos redox celulares.


DCFH-DA, diacetato de 2′,7′-diclorodihidrofluoresceína (también llamado diacetato de 2′,7′-diclorofluorescina), es hidrolizado por esterasas celulares a 2′,7′-diclorodihidrofluoresceína (también llamada 2′,7′-diclorofluorescina) y luego se oxida a 2′,7′-diclorofluoresceína principalmente por H2O2.

El diacetato de 2′,7′-diclorodihidrofluoresceína podría ser reactivo frente a una amplia gama de reacciones oxidantes que pueden aumentar durante el estrés oxidativo intracelular. Esta sonda es ampliamente utilizada para monitorear procesos redox celulares.

Nombre en Ingles: DCFH-DA [2′,7′-Dichlorodihydrofluorescein diacetate] *CAS 4091-99-0*

AAT-15204DCFH-DA [diacetato de 2′,7′-diclorodihidrofluoresceína]25 mg

Importante: Producto no diseñado para uso diagnóstico o terapéutico. Solo para uso en investigación (RUO). Almacenamiento a largo plazo: Congelacion a < -15 °C. Minimizar la exposición a la luz.


Microscopio de Fluorescencia

ExcitaciónJuego de filtros FITC
EmisiónJuego de filtros FITC
Placa RecomendadaPared negra/fondo claro
Especificaciones instrumentoJuego de filtros FITC

Propiedades Fisicas

Peso Molecular 487.29


Abrir en Advanced Spectrum Viewer

Propiedades Espectrales

Excitación (nm)505
Emisión (nm)526


Preparación de la solución de stock común
Volumen de DMSO necesario para reconstituir la masa específica de DCFH-DA [diacetato de 2′,7′-diclorodihidrofluoresceína] CAS 4091-99-0 a la concentración dada. Tenga en cuenta que el volumen es solo para preparar la solución madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales/fisiológicos apropiados.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM205.217 µL1.026 mL2.052 mL10.261 mL20.522 mL
5 mM41.043 µL205.217 µL410.433 µL2.052 mL4.104 mL
10 mM20.522 µL102.608 µL205.217 µL1.026 mL2.052 mL

Figura 1. Estructura quimica para DCFH-DA [2′,7′-Dichlorodihydrofluorescein diacetate] *CAS 4091-99-0*

Informacion Importante

Este material se vende únicamente con fines de investigación y no es necesario que aparezca en el inventario de TSCA. Este prodcuto no está diseñado para uso alimentario, farmacéutico, doméstico, agrícola o cosmético. Su uso debe ser supervisado por un persona técnicamente calificada con experiencia en el manejo de productos químicos potencialmente peligrosos. Lo anterior se cree información correcta, pero no pretende ser unica ni completa y se utilizará solo como una guía.

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