CytoTrace™ Green CMFDA sonda fluorescente conserva las propiedades espectrales de FITC, por lo que su señal normalmente se puede monitorear mediante microscopía de fluorescencia o citometría de flujo.
Descripción
CytoTrace™ Green CMFDA, sonda fluorescente
CytoTrace™ Green CMFDA es un excelente reemplazo de CellTracker™ Green CMFDA (Invitrogen C2925, CellTracker™ es la marca comercial de Invitrogen).
La sonda fluorescente CMFDA conserva las propiedades espectrales de FITC, por lo que su señal normalmente se puede monitorear mediante microscopía de fluorescencia o citometría de flujo. Pasa libremente a través de las membranas celulares y se convierte en productos impermeables a las células al reaccionar con los componentes celulares.
El producto de la reacción impermeable a las células pasa a las células hijas a lo largo de varias generaciones, pero no se transfiere a las células adyacentes de la población. Las células cargadas con la sonda Cytotrace™ Green suelen ser fluorescentes y viables durante al menos 24 horas, lo que convierte a esta sonda en un excelente rastreador celular a largo plazo.
El patrón de tinción se puede fijar con formaldehído o glutaraldehído para amplificación de señal y otras aplicaciones.
Nombre en Ingles: CytoTrace™ Green CMFDA
| Catalogo | Producto | Presentación |
|---|---|---|
| AAT-22017 | CytoTrace™ Green CMFDA, sonda fluorescente | 1 mg |
Importante: Solo para uso en investigación (RUO). Almacenamiento a <-15 °C. Minimizar exposición a la luz. Material se entrega en geles.
Espectro
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Propiedades Espectrales
| Excitación (nm) | 498 |
| Emisión (nm) | 517 |
Calculadora
Preparación de solución de stock común
Tabla 1. Volumen de DMSO necesario para reconstituir la masa específica de CytoTrace™ Green CMFDA a una concentración determinada. Tenga en cuenta que el volumen es sólo para preparar la solución madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales/fisiológicos adecuados.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 215.119 µL | 1.076 mL | 2.151 mL | 10.756 mL | 21.512 mL |
| 5 mM | 43.024 µL | 215.119 µL | 430.237 µL | 2.151 mL | 4.302 mL |
| 10 mM | 21.512 µL | 107.559 µL | 215.119 µL | 1.076 mL | 2.151 mL |
Imagen
Figura 1. Estructura quimica de CytoTrace™ Green CMFDA
Productos Relacionados
| CytoTrace™ Orange CMTMR *CAS 323192-14-9* |
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| CytoTrace™ Red CFDA |
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| CytoTrace™ CM-DiD |
Bibliografia
Biodegradable Mg alloy modified with bioactive exosomes for cardiovascular stent application
Authors: Hou, Ya-chen and Li, Jing-an and Cao, Chang and Su, Chang and Qin, Zhen and Zhang, Ge and Guo, Jia-cheng and Tang, Jun-nan and Zhang, Jin-ying and Guan, Shao-kang
Journal: Journal of Magnesium and Alloys (2023)
An integrated strategy combining network toxicology and feature-based molecular networking for exploring hepatotoxic constituents and mechanism of Epimedii Folium-induced hepatotoxicity in vitro
Authors: Zhao, Zhen and Du, Jin-Fa and Wang, Qiao-Lei and Qiu, Fang-Ning and Chen, Xu-Yan and Liu, Feng-Jie and Li, Ping and Jiang, Yan and Li, Hui-Jun
Journal: Food and Chemical Toxicology (2023): 113785
A promoting nitric oxide-releasing coating containing copper ion on ZE21B alloy for potential vascular stent application
Authors: Jia, Qianying and Jia, Qinggong and Zhu, Shijie and Zheng, Yufeng and Mine, Yoji and Takashima, Kazuki and Guan, Shaokang
Journal: Journal of Magnesium and Alloys (2022)
The increased ratio of Mg2+/Ca2+ from degrading magnesium alloys directs macrophage fate for functionalized growth of endothelial cells
Authors: Hou, Ya-chen and Witte, Frank and Li, Jingan and Guan, Shaokang
Journal: Smart Materials in Medicine (2022)
Clinical Strains of Helicobacter pylori With Strong Cell Invasiveness and the Protective Effect of Patchouli Alcohol by Improving miR-30b/C Mediated Xenophagy
Authors: Xu, Yifei and Deng, Qiuhua and Zhong, Yuanzun and Jing, Li and Li, Haiwen and Li, Jingwei and Yu, Huimin and Pan, Huafeng and Guo, Shaoju and Cao, Hongying and others,
Journal: Frontiers in Pharmacology (2021): 1037
Inflation-collapse dynamics drive patterning and morphogenesis in intestinal organoids
Authors: Tallapragada, Naren P and Cambra, Hailey M and Wald, Tomas and Jalbert, Samantha Keough and Abraham, Diana M and Klein, Ophir D and Klein, Allon M
Journal: Cell Stem Cell (2021)
Referencias
Ver todas las 23 referencias: Citation Explorer
Fluorescence-Based Transport Assays Revisited in a Human Renal Proximal Tubule Cell Line
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Journal: Mol Pharm (2016): 933
The variable chemotherapeutic response of Malabaricone-A in leukemic and solid tumor cell lines depends on the degree of redox imbalance
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Journal: Phytomedicine (2015): 713
Cell membrane tracker based on restriction of intramolecular rotation
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Journal: ACS Appl Mater Interfaces (2014): 8971
Evaluation of stability and sensitivity of cell fluorescent labels when used for cell migration
Authors: Beem E, Segal MS.
Journal: J Fluoresc (2013): 975
A multiple model probability hypothesis density tracker for time-lapse cell microscopy sequences
Authors: Rezatofighi SH, Gould S, Vo BN, Mele K, Hughes WE, Hartley R.
Journal: Inf Process Med Imaging (2013): 110
TLM-Tracker: software for cell segmentation, tracking and lineage analysis in time-lapse microscopy movies
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Journal: Bioinformatics (2012): 2276
The interplay between Leishmania promastigotes and human Natural Killer cells in vitro leads to direct lysis of Leishmania by NK cells and modulation of NK cell activity by Leishmania promastigotes
Authors: Lieke T, Nylen S, Eidsmo L, Schmetz C, Berg L, Akuffo H.
Journal: Parasitology (2011): 1898
The exocytosis of fluorescent nanodiamond and its use as a long-term cell tracker
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Horizontal DNA transfer from donor to host cells as an alternative mechanism of epithelial chimerism after allogeneic hematopoietic cell transplantation
Authors: Waterhouse M, Themeli M, Bertz H, Zoumbos N, Finke J, Spyridonidis A.
Journal: Biol Blood Marrow Transplant (2011): 319
Overlap-Based Cell Tracker
Authors: Chalfoun J, Cardone A, Dima AA, Allen DP, Halter MW.
Journal: J Res Natl Inst Stand Technol (2010): 477
Application Notes
Multiplexing Cell Proliferation and Cytotoxicity Assays Using Calcein Red and CytoCalceins
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Abbreviation of Common Chemical Compounds Related to Peptides
Annexin V
FAQ
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Can 7-AAD be fixed?
Question Can 7-AAD stain live cells?
Can Cy3 be used in flow cytometry?
AssayWise
HRP Antibody Labeling Using Buccutite™ Crosslinking Technology
iFluor® 700 Dyes
Hydroxyl Radical Detection
Buccutite™ Conjugation Kits: Quick and Easy Antibody Labeling
Nucleic Acid Detection, Quantification and Imaging