CytoTrace™ Green CMFDA, sonda fluorescente


CytoTrace™ Green CMFDA sonda fluorescente conserva las propiedades espectrales de FITC, por lo que su señal normalmente se puede monitorear mediante microscopía de fluorescencia o citometría de flujo.


CytoTrace™ Green CMFDA, sonda fluorescente

CytoTrace™ Green CMFDA es un excelente reemplazo de CellTracker™ Green CMFDA (Invitrogen C2925, CellTracker™ es la marca comercial de Invitrogen).

La sonda fluorescente CMFDA conserva las propiedades espectrales de FITC, por lo que su señal normalmente se puede monitorear mediante microscopía de fluorescencia o citometría de flujo. Pasa libremente a través de las membranas celulares y se convierte en productos impermeables a las células al reaccionar con los componentes celulares.

El producto de la reacción impermeable a las células pasa a las células hijas a lo largo de varias generaciones, pero no se transfiere a las células adyacentes de la población. Las células cargadas con la sonda Cytotrace™ Green suelen ser fluorescentes y viables durante al menos 24 horas, lo que convierte a esta sonda en un excelente rastreador celular a largo plazo.

El patrón de tinción se puede fijar con formaldehído o glutaraldehído para amplificación de señal y otras aplicaciones.

Nombre en Ingles: CytoTrace™ Green CMFDA

AAT-22017CytoTrace™ Green CMFDA, sonda fluorescente1 mg

Importante: Solo para uso en investigación (RUO). Almacenamiento a <-15 °C. Minimizar exposición a la luz. Material se entrega en geles.


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Propiedades Espectrales

Excitación (nm)498
Emisión (nm)517


Preparación de solución de stock común
Tabla 1. Volumen de DMSO necesario para reconstituir la masa específica de CytoTrace™ Green CMFDA a una concentración determinada. Tenga en cuenta que el volumen es sólo para preparar la solución madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales/fisiológicos adecuados.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM215.119 µL1.076 mL2.151 mL10.756 mL21.512 mL
5 mM43.024 µL215.119 µL430.237 µL2.151 mL4.302 mL
10 mM21.512 µL107.559 µL215.119 µL1.076 mL2.151 mL


Figura 1. Estructura quimica de CytoTrace&trade; Green CMFDA

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Application Notes

Multiplexing Cell Proliferation and Cytotoxicity Assays Using Calcein Red and CytoCalceins
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Abbreviation of Common Chemical Compounds Related to Peptides
Annexin V


Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Can 7-AAD be fixed?
Question Can 7-AAD stain live cells?
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