El éster CytoCalcein™ Violet 660 AM está diseñado para marcar células vivas de la misma manera que lo hace Calcein, AM.
Descripción
CytoCalcein™ Violet 660, sodium salt *Excited at 405 nm*
CytoCalcein™ Violet 660 es el producto escindido por esterasa de CytoCalcein™ Violet 660 AM en células vivas. CytoCalcein™ Violet 660 no es permeable a las células. Es el producto de referencia para CytoCalcein™ Violet 660 AM que está diseñado para marcar células vivas de la misma manera que lo hace Calcein, AM.
CytoCalcein™ Violet 660 se excita bien con el láser Violet a 405 nm, que ahora está equipado en la mayoría de los citómetros de flujo. También está bien excitado por las fuentes de excitación de los microscopios de fluorescencia. Esta separación espectral excepcional de los fluoróforos FACS típicos existentes proporciona opciones adicionales para experimentos de multiplexación con un citómetro de flujo o un microscopio de fluorescencia.
CytoCalcein™ Violet 660 se ha desarrollado para aplicaciones de citometría de flujo y de imágenes de fluorescencia de multiplexación. Proporciona un nuevo color único para el análisis de citometría de flujo de células vivas.
Nombre en Ingles: CytoCalcein™ Violet 660, sodium salt *Excited at 405 nm*
| Catalogo | Producto | Presentación |
|---|---|---|
| AAT-21903 | CytoCalcein™ Violet 660, sodium salt *Excited at 405 nm* | 1mg |
Importante, Solo para uso en investigación (RUO). Almacenamiento a largo plazo: Congelar a < -15 °C. Minimizar la exposición a la luz.
Propiedades Fisicas
| Peso Molecular | 662.63 |
| Solvente | DMSO |
Calculadora
Preparación de solución de Stock común
Tabla 1. Volumen de DMSO necesario para reconstituir la masa específica de CytoCalcein™ Violet 660, sal sódica Excitada a 405 nm hasta la concentración dada. Tenga en cuenta que el volumen es sólo para preparar la solución madre. Consulte el protocolo experimental de muestra para conocer los tampones experimentales/fisiológicos adecuados.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 150.914 µL | 754.569 µL | 1.509 mL | 7.546 mL | 15.091 mL |
| 5 mM | 30.183 µL | 150.914 µL | 301.828 µL | 1.509 mL | 3.018 mL |
| 10 mM | 15.091 µL | 75.457 µL | 150.914 µL | 754.569 µL | 1.509 mL |
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Application notes
Annexin V
Experimental Protocol for Calcein AM Assay
Multi-Color Labeling and Functional Analysis of Live Cells Using Fluorescent Calcein AM Dyes
Multiplexing Cell Proliferation and Cytotoxicity Assays Using Calcein Red and CytoCalceins
Calcein
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