Cyanine 7 bissuccinimidyl ester, potassium salt

[same as GE Cy7® bisNHS ester]

Descripción

Este colorante Cy7® es la misma molécula que el éster bisNHS Cy7® de GE. Reacciona fácilmente con grupos amino. Tiene dos grupos NHS que podrían dar una señal más brillante en algunas proteínas que el éster mono NHS Cy7 (n.º 161 y n.º 290).

Nuestros Cy7® Fluors se someten a pruebas de control de calidad exhaustivas para garantizar altos niveles de cromóforo y contenido de colorante reactivo. Se ha utilizado una variedad de tintes de cianina 7 (Cy7®) para marcar moléculas biológicas para imágenes de fluorescencia y otros análisis bioquímicos basados en fluorescencia. Se utilizan ampliamente para marcar péptidos, proteínas y oligos, etc. Los conjugados de colorante Cy7® son uno de los fluoróforos rojos del infrarrojo cercano más comunes utilizados en aplicaciones de imágenes in vivo. El éster Cy7® NHS reacciona fácilmente con los grupos amino. Cy7® es la marca comercial de GE Healthcare.

CatalogoProductoPresentacion
AAT-295Cyanine 7 bissuccinimidyl ester, potassium salt [same as GE Cy7® bisNHS ester]1mg

Importante: Solo para uso en investigación (RUO). Almacenamiento: Congelación (< -15 °C). Minimizar la exposición a la luz.

Propiedades Fisicas

Peso Molecular 1001.17
DisolventeDMSO

Espectro

Abrir en Advanced Spectrum Viewer

Propiedades espectrales

Factor de corrección (260 nm)0.05
Factor de corrección (280 nm)0.036
Factor de corrección (482 nm)0.0005
Factor de corrección (565 nm)0.0193
Factor de corrección (650 nm)0.165
Coeficiente de extinción (cm -1 M -1)250000
Excitación (nm)756
Emisión (nm)779
Rendimiento cuántico0.3

Calculadora

Preparación de la solución de stock común

Volumen de DMSO necesario para reconstituir la masa específica de Cianina 7 bissuccinimidil éster, sal de potasio [igual que GE Cy7® bisNHS éster] a la concentración dada. Tenga en cuenta que el volumen es solo para preparar la solución madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales/fisiológicos apropiados.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM99.883 µL499.416 µL998.831 µL4.994 mL9.988 mL
5 mM19.977 µL99.883 µL199.766 µL998.831 µL1.998 mL
10 mM9.988 µL49.942 µL99.883 µL499.416 µL998.831 µL

Figura 1.

Estructura química del éster de cianina 7 bissuccinimidilo, sal de potasio [igual que el éster GE Cy7® bisNHS]

Productos Similares

NombreExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)Correction Factor (482 nm)Correction Factor (565 nm)
Cyanine 3 bissuccinimidyl ester, potassium salt [same as GE Cy3® bisNHS ester]55556915000010.1510.070.073
Cyanine 5 bissuccinimidyl ester, potassium salt [same as GE Cy5® bisNHS ester]65167025000010.271, 0.420.020.030.0090.09
Cyanine 7 monosuccinimidyl ester, potassium salt [same as GE Cy7® NHS ester]7567792500000.30.050.0360.00050.0193

Bibliografía

Thermo-sensitive hydrogel PLGA-PEG-PLGA as a vaccine delivery system for intramuscular immunization
Authors: Wang, Xiaoyan and Zhang, Yu and Xue, Wei and Wang, Hong and Qiu, Xiaozhong and Liu, Zonghua
Journal: Journal of Biomaterials Applications (2017): 923–932

Cube-shaped theranostic paclitaxel prodrug nanocrystals with surface functionalization of SPC and MPEG-DSPE for imaging and chemotherapy
Authors: Guo, Fuqiang and Shang, Jiajia and Zhao, Hai and Lai, Kangrong and Li, Yang and Fan, Zhongxiong and Hou, Zhenqing and Su, Guanghao
Journal: Colloids and Surfaces B: Biointerfaces (2017)

Light/magnetic hyperthermia triggered drug released from multi-functional thermo-sensitive magnetoliposomes for precise cancer synergetic theranostics
Authors: Guo, Yuxin and Zhang, Yang and Ma, Jinyuan and Li, Qi and Li, Yang and Zhou, Xinyi and Zhao, Dan and Song, Hua and Chen, Qing and Zhu, Xuan
Journal: Journal of Controlled Release (2017)

Molecular Basis and Consequences of the Cytochrome c-tRNA Interaction
Authors: Liu, Cuiping and Stonestrom, Aaron J and Christian, Thomas and Yong, Jeongsik and Takase, Ryuichi and Hou, Ya-Ming and Yang, Xiaolu
Journal: Journal of Biological Chemistry (2016): 10426–10436

Click-electron microscopy for imaging metabolically tagged nonprotein biomolecules
Authors: Ngo, John T and Adams, Stephen R and Deerinck, Thomas J and Boassa, Daniela and Rodriguez-Rivera, Frances and Palida, Sakina F and Bertozzi, Carolyn R and Ellisman, Mark H and Tsien, Roger Y
Journal: Nat Chem Biol (2016): 459–465

Design, synthesis and evaluation of VEGF-siRNA/CRS as a novel vector for gene delivery
Authors: Zhao, Wen and Zhang, Yifan and Jiang, Xueyun and Cui, Chunying
Journal: Drug Design, Development and Therapy (2016): 3851

Affinity-Controlled Protein Encapsulation into Sub-30 nm Telodendrimer Nanocarriers by Multivalent and Synergistic Interactions
Authors: Wang, Xu and Shi, Changying and Zhang, Li and Bodman, Alexa and Guo, D and an , undefined and Wang, Lili and Hall, Walter A and Wilkens, Stephan and Luo, Juntao
Journal: Biomaterials (2016)

Carboxymethyl Dextran-Stabilized Polyethylenimine-Poly (epsilon-caprolactone) Nanoparticles-Mediated Modulation of MicroRNA-34a Expression via Small-Molecule Modulator for Hepatocellular Carcinoma Therapy
Authors: Deng, Xiongwei and Yin, Zhaoxia and Zhou, Zhixiang and Wang, Yihui and Zhang, Fang and Hu, Qin and Yang, Yishu and Lu, Jianqing and Wu, Yan and Sheng, Wang and others, undefined
Journal: ACS applied materials & interfaces (2016): 17068–17079

Multiplexed single-cell in situ RNA analysis by reiterative hybridization
Authors: Xiao, Lu and Guo, Jia
Journal: Analytical Methods (2015): 7290–7295

Determination of the active transport of fucoidan derived from okinawa mozuku across the human intestinal caco-2 cells as assessed by size-exclusion chromatography
Authors: Nagamine, Takeaki and Hayakawa, Kou and Nakazato, Kyoumi and Iha, Masahiko
Journal: Journal of Chromatography B (2015): 187–193

Referencias

Ver todas las 21 referencias: Citation Explorer

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Journal: Appl Phys Lett (2011): 143703

Theranostic cRGD-BioShuttle Constructs Containing Temozolomide- and Cy7 For NIR-Imaging and Therapy
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Journal: Theranostics (2011): 381

Rational approach to select small peptide molecular probes labeled with fluorescent cyanine dyes for in vivo optical imaging
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Journal: Biochemistry (2011): 2691

In vivo detection of embryonic stem cell-derived cardiovascular progenitor cells using Cy3-labeled Gadofluorine M in murine myocardium
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Quantitative proteomics by fluorescent labeling of cysteine residues using a set of two cyanine-based or three rhodamine-based dyes
Authors: Volke D, Hoffmann R.
Journal: Electrophoresis (2008): 4516

Surface plasmon resonance-enhanced fluorescence implementation of a single-step competition assay: demonstration of fatty acid measurement using an anti-fatty acid monoclonal antibody and a Cy5-labeled fatty acid
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Thiazole orange and Cy3: improvement of fluorescent DNA probes with use of short range electron transfer
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Near-infrared fluorescence imaging of tumor integrin alpha v beta 3 expression with Cy7-labeled RGD multimers
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Cy7-Bis-dipicolylamine-zinc
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Cy7-Tetrameric arginine-glycine-aspartic acid peptide
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Application Notes (en Ingles)

A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)