Calcein UltraBlue™ AM, sonda fluorescente


Calcein UltraBlue™ AM es una versión permeable a las células de Calcein UltraBlue™


Calcein UltraBlue™ AM, sonda fluorescente

Calcein UltraBlue™ AM es una versión permeable a las células de Calcein UltraBlue™. Al ingresar a las células vivas, Calcein UltraBlue™ AM, débilmente fluorescente, se hidroliza en Calcein UltraBlue™, que tiene máximos de excitación/emisión similares a los de Calcein Blue, DAPI, Hoechst y AMCA.

Esta excepcional separación espectral de los típicos fluoróforos verdes y rojos (como FITC, TMR y Texas Red) proporciona opciones adicionales para experimentos de multiplexación. Calcein UltraBlue™ tiene propiedades espectrales similares a las de Calcein Blue.

Sin embargo, en comparación con Calcein Blue, Calcein UltraBlue™ tiene una mayor fotoestabilidad y una mayor intensidad de fluorescencia a pH fisiológico, lo que la convierte en una sonda fluorescente más robusta que Calcein Blue.

Nombre en Ingles: Calcein UltraBlue™ AM

AAT-21908Calcein UltraBlue™ AM10 x 50 ug

Importante, Solo para uso en investigación (RUO). Almacenamiento a largo plazo: Congelar a  < -15 °C. Minimizar la exposición a la luz.


Citómetro de flujo

Excitación350/405 nm laser
Emisión450/40 nm filter
Especificaciones InstrumentoPacific Blue channel

Microscopio de Fluorescencia

ExcitaciónJuego de filtros DAPI
EmisiónJuego de filtros DAPI
Placa recomendadaPared negra/fondo claro

Lector de fluorescencia de microplacas

Placa recomendadaNegra, solida
Especificaciones InstrumentoModo lectura inferior


Abrir en Advanced Spectrum Viewer

Propiedades Espectrales

Excitación (nm)359
Emisión (nm)458

Propiedades Fisicas

Peso Molecular802.68


Preparación de solución madre común
Tabla 1. Volumen de agua necesario para reconstituir la masa específica de sal sódica de Calcein UltraBlue™ a una concentración determinada. Tenga en cuenta que el volumen es sólo para preparar la solución madre. Consulte el protocolo experimental de muestra para conocer los tampones experimentales/fisiológicos adecuados.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM124.583 µL622.913 µL1.246 mL6.229 mL12.458 mL
5 mM24.917 µL124.583 µL249.165 µL1.246 mL2.492 mL
10 mM12.458 µL62.291 µL124.583 µL622.913 µL1.246 mL


Figura 1: Imágenes de fluorescencia de células HeLa teñidas con Calcein UltraBlue™ AM (fila superior) o Calcein Blue AM (fila inferior) en una placa de 96 pocillos de pared negra Costar/fondo transparente. Izquierda: células HeLa vivas en buffer HH; Medio: células HeLa vivas en medio; Derecha: células HeLa fijas.

Productos Relacionados

NameExcitation (nm)Emission (nm)
Calcein Red™ AM562576
Calcein Blue, AM *CAS 168482-84-6*354441
Calcein UltraGreen™ AM492514

Otros Productos

Calcein Deep Red™ acetate
Calcein Deep Red™ AM ester
Calcein Red™ sodium salt
Calcein UltraBlue™ AM
Calcein UltraBlue™ sodium salt
Calcein *UltraPure Grade* *CAS 207124-64-9*
Calcein, AM *CAS 148504-34-1*
Calcein, AM *UltraPure grade* *CAS 148504-34-1*
Calcein Blue *CAS 54375-47-2*
Calcein Blue, AM *CAS 168482-84-6*
Calcein Orange™ sodium salt
Calcein Orange™ diacetate
CytoCalcein™ Violet 450 *Excited at 405 nm*
CytoCalcein™ Violet 500 *Excited at 405 nm*
Calcein UltraGreen™ AM
CytoCalcein™ Violet 660, sodium salt *Excited at 405 nm*
CytoCalcein™ Violet 660, AM *Excited at 405 nm*


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Application notes

Experimental Protocol for Calcein AM Assay
Annexin V
Calcein AM Assay Standardization
Multi-Color Labeling and Functional Analysis of Live Cells Using Fluorescent Calcein AM Dyes


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