Calcein UltraBlue™ AM es una versión permeable a las células de Calcein UltraBlue™
Descripción
Calcein UltraBlue™ AM, sonda fluorescente
Calcein UltraBlue™ AM es una versión permeable a las células de Calcein UltraBlue™. Al ingresar a las células vivas, Calcein UltraBlue™ AM, débilmente fluorescente, se hidroliza en Calcein UltraBlue™, que tiene máximos de excitación/emisión similares a los de Calcein Blue, DAPI, Hoechst y AMCA.
Esta excepcional separación espectral de los típicos fluoróforos verdes y rojos (como FITC, TMR y Texas Red) proporciona opciones adicionales para experimentos de multiplexación. Calcein UltraBlue™ tiene propiedades espectrales similares a las de Calcein Blue.
Sin embargo, en comparación con Calcein Blue, Calcein UltraBlue™ tiene una mayor fotoestabilidad y una mayor intensidad de fluorescencia a pH fisiológico, lo que la convierte en una sonda fluorescente más robusta que Calcein Blue.
Nombre en Ingles: Calcein UltraBlue™ AM
| Catalogo | Producto | Presentación |
|---|---|---|
| AAT-21908 | Calcein UltraBlue™ AM | 10 x 50 ug |
Importante, Solo para uso en investigación (RUO). Almacenamiento a largo plazo: Congelar a < -15 °C. Minimizar la exposición a la luz.
Plataforma
Citómetro de flujo
| Excitación | 350/405 nm laser |
| Emisión | 450/40 nm filter |
| Especificaciones Instrumento | Pacific Blue channel |
Microscopio de Fluorescencia
| Excitación | Juego de filtros DAPI |
| Emisión | Juego de filtros DAPI |
| Placa recomendada | Pared negra/fondo claro |
Lector de fluorescencia de microplacas
| Excitación | 360 |
| Emisión | 450 |
| Cutoff | 420 |
| Placa recomendada | Negra, solida |
| Especificaciones Instrumento | Modo lectura inferior |
Espectro
Abrir en Advanced Spectrum Viewer
Propiedades Espectrales
| Excitación (nm) | 359 |
| Emisión (nm) | 458 |
Propiedades Fisicas
| Peso Molecular | 802.68 |
| Solvente | DMSO |
Calculadora
Preparación de solución madre común
Tabla 1. Volumen de agua necesario para reconstituir la masa específica de sal sódica de Calcein UltraBlue™ a una concentración determinada. Tenga en cuenta que el volumen es sólo para preparar la solución madre. Consulte el protocolo experimental de muestra para conocer los tampones experimentales/fisiológicos adecuados.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 124.583 µL | 622.913 µL | 1.246 mL | 6.229 mL | 12.458 mL |
| 5 mM | 24.917 µL | 124.583 µL | 249.165 µL | 1.246 mL | 2.492 mL |
| 10 mM | 12.458 µL | 62.291 µL | 124.583 µL | 622.913 µL | 1.246 mL |
Imagenes
Figura 1: Imágenes de fluorescencia de células HeLa teñidas con Calcein UltraBlue™ AM (fila superior) o Calcein Blue AM (fila inferior) en una placa de 96 pocillos de pared negra Costar/fondo transparente. Izquierda: células HeLa vivas en buffer HH; Medio: células HeLa vivas en medio; Derecha: células HeLa fijas.
Productos Relacionados
| Name | Excitation (nm) | Emission (nm) |
| Calcein Red™ AM | 562 | 576 |
| Calcein Blue, AM *CAS 168482-84-6* | 354 | 441 |
| Calcein UltraGreen™ AM | 492 | 514 |
Otros Productos
Bibliografia
Ver todas las 18 bibliografias: Citation Explorer
Double emulsion picoreactors for high-throughput single-cell encapsulation and phenotyping via FACS
Authors: Brower, Kara K and Khariton, Margarita and Suzuki, Peter H and Still, Chris and Kim, Gaeun and Calhoun, Suzanne GK and Qi, Lei S and Wang, Bo and Fordyce, Polly M
Journal: Analytical chemistry (2020): 13262–13270
Functional imaging of neuronal activity of auditory cortex by using Cal-520 in anesthetized and awake mice
Authors: Li, Jingcheng and Zhang, Jianxiong and Wang, Meng and Pan, Junxia and Chen, Xiaowei and Liao, Xiang
Journal: Biomedical Optics Express (2017): 2599–2610
NINJ2–A novel regulator of endothelial inflammation and activation
Authors: Wang, Jingjing and Fa, Jingjing and Wang, Pengyun and Jia, Xinzhen and Peng, Huixin and Chen, Jing and Wang, Yifan and Wang, Chenhui and Chen, Qiuyun and Tu, Xin and others, undefined
Journal: Cellular Signalling (2017)
Influence of hypothermia and subsequent rewarming upon leukocyte-endothelial interactions and expression of Junctional-Adhesion-Molecules A and B
Authors: Bogert, Nicolai V and Werner, Isabella and Kornberger, Angela and Meybohm, Patrick and Moritz, Anton and Keller, Till and Stock, Ulrich A and Beiras-Fern, undefined and ez, Andres
Journal: Scientific reports (2016)
Inhibition of ABC transport proteins by oil sands process affected water
Authors: Alharbi, Hattan A and Saunders, David MV and Al-Mousa, Ahmed and Alcorn, Jane and Pereira, Alberto S and Martin, Jonathan W and Giesy, John P and Wiseman, Steve B
Journal: Aquatic Toxicology (2016): 81–88
Rapid generation of collagen-based microtissues to study cell–matrix interactions
Authors: Brett, Marie-Elena and Crampton, Alex and ra L , undefined and Wood, David K
Journal: Technology (2016): 1–8
Toxicokinetics and toxicodynamics of chlorpyrifos is altered in embryos of Japanese medaka exposed to oil sands process-affected water: evidence for inhibition of P-glycoprotein
Authors: Alharbi, Hattan A and Alcorn, Jane and Al-Mousa, Ahmed and Giesy, John P and Wiseman, Steve B
Journal: Journal of Applied Toxicology (2016)
Flexible Endoscopic Spray Application of Respiratory Epithelial Cells as Platform Technology to Apply Cells in Tubular Organs
Authors: Thiebes, Anja Lena and Reddemann, Manuel Armin and Palmer, Johannes and Kneer, Reinhold and Jockenhoevel, Stefan and Cornelissen, Christian Gabriel
Journal: Tissue Engineering Part C: Methods (2016): 322–331
Erythropoietin Stimulates Endothelial Progenitor Cells to Induce Endothelialization in an Aneurysm Neck After Coil Embolization by Modulating Vascular Endothelial Growth Factor
Authors: Liu, Peixi and Zhou, Yingjie and An, Qingzhu and Song, Yaying and Chen, Xi and Yang, Guo-Yuan and Zhu, Wei
Journal: MEDICINE (2016): 1–8
Spraying respiratory epithelial cells to coat tissue-engineered constructs
Authors: Thiebes, Anja Lena and Albers, Stefanie and Klopsch, Christian and Jockenhoevel, Stefan and Cornelissen, Christian G
Journal: BioResearch open access (2015): 278–287
Referencias
Ver todas las 84 referencias: Citation Explorer
Functional evidence that the self-renewal gene NANOG regulates esophageal squamous cancer development
Authors: Li, Deng and Xiang, Xiaocong and Yang, Fei and Xiao, Dongqin and Liu, Kang and Chen, Zhu and Zhang, Ruolan and Feng, Gang
Journal: Biochemical and Biophysical Research Communications (2017)
Localized functional chemical stimulation of TE 671 cells cultured on nanoporous membrane by calcein and acetylcholine
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A vaccination and challenge model using calcein marked fish
Authors: Klesius PH, Evans JJ, Shoemaker CA, Pasnik DJ.
Journal: Fish Shellfish Immunol (2006): 20
Novel fluorescence assay using calcein-AM for the determination of human erythrocyte viability and aging
Authors: Bratosin D, Mitrofan L, Palii C, Estaquier J, Montreuil J.
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Cytotoxic effects of 100 reference compounds on Hep G2 and HeLa cells and of 60 compounds on ECC-1 and CHO cells. I mechanistic assays on ROS, glutathione depletion and calcein uptake
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Journal: Toxicol In Vitro (2005): 505
Calcein AM release-based cytotoxic cell assay for fish leucocytes
Authors: Iwanowicz LR, Densmore CL, Ottinger CA.
Journal: Fish Shellfish Immunol (2004): 127
Calcein-AM is a detector of intracellular oxidative activity
Authors: Uggeri J, Gatti R, Belletti S, Sc and roglio R, Corradini R, Rotoli BM, Orl and ini G., undefined
Journal: Histochem Cell Biol (2004): 499
Comparison of the usefulness of the MTT, ATP, and calcein assays to predict the potency of cytotoxic agents in various human cancer cell lines
Authors: Mueller H, Kassack MU, Wiese M.
Journal: J Biomol Screen (2004): 506
In vitro assay of mineralized-tissue formation on titanium using fluorescent staining with calcein blue
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Journal: Biomaterials (2003): 3885
The effects of calcium chloride and sodium chloride on the electroporation-mediated skin permeation of fluorescein isothiocyanate (FITC)-dextrans in vitro
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Application notes
Experimental Protocol for Calcein AM Assay
Annexin V
Calcein
Calcein AM Assay Standardization
Multi-Color Labeling and Functional Analysis of Live Cells Using Fluorescent Calcein AM Dyes
FAQ
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