Calcein Deep Red. Calcein AM es una de las sondas fluorescentes más populares que se utilizan para marcar y monitorear las funciones celulares de células vivas.
Descripción
Calcein Deep Red™
Calcein AM es una de las sondas fluorescentes más populares que se utilizan para marcar y controlar las funciones celulares de células vivas.
Sin embargo, el color único de Calcein AM hace imposible utilizar este valioso reactivo en aplicaciones multicolores. Por ejemplo, es imposible utilizar Calcein AM en células transfactadas con GFP debido al mismo color que GFP. Para abordar esta limitación de color de Calcein AM, hemos desarrollado Calcein Orange™, Calcein Red™ y Calcein Deep Red™.
Estos nuevos análogos de Calcein AM permiten el etiquetado multicolor y el análisis funcional de células vivas en combinación con Calcein AM. Calcein Deep Red™ es el producto hidrolizado con esterasa del acetato de Calcein Deep Red™ en las células.
El tinte Calcein Deep Red™ se puede controlar con el juego de filtros Cy5 común. Se utiliza como estándar de referencia de fluorescencia para el acetato de Calcein Deep Red™.
Nombre en Ingles: Calcein Deep Red™
| Catalogo | Producto | Presentación |
|---|---|---|
| AAT-21902 | Calcein Deep Red™ | 1 mg |
Importante, Solo para uso en investigación (RUO). Almacenamiento a largo plazo: Congelar a < -15 °C. Minimizar la exposición a la luz.
Plataforma
Citómetro de flujo
| Excitación | laser 640 nm |
| Emisión | filtro 660/20 nm |
| Especificaciones Instrumento | canal APC |
Microscopio de Fluorescencia
| Excitación | Juego de filtros Cy5 |
| Emisión | Juego de filtros Cy5 |
| Placa recomendada | Pared negra/fondo claro |
Lector de fluorescencia de microplacas
| Excitación | 620 |
| Emisión | 660 |
| Cutoff | 630 |
| Placa recomendada | Negra, solida |
Espectro
Abrir en Advanced Spectrum Viewer
Propiedades Espectrales
| Excitación (nm) | 643 |
| Emisión (nm) | 663 |
Propiedades Fisicas
| Peso Molecular | 373.66 |
| Solvente | DMSO |
Calculadora
Preparación de solución madre común
Tabla 1. Volumen de agua necesario para reconstituir la masa específica de sal sódica de Calcein Deep Red™ a una concentración determinada. Tenga en cuenta que el volumen es sólo para preparar la solución madre. Consulte el protocolo experimental de muestra para conocer los tampones experimentales/fisiológicos adecuados.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 267.623 µL | 1.338 mL | 2.676 mL | 13.381 mL | 26.762 mL |
| 5 mM | 53.525 µL | 267.623 µL | 535.246 µL | 2.676 mL | 5.352 mL |
| 10 mM | 26.762 µL | 133.811 µL | 267.623 µL | 1.338 mL | 2.676 mL |
Imagenes
Figura 1: Imágenes de células HeLa vivas teñidas con Calcein Deep Red™ (Cat.#21902) y Calcein Deep Red™ AM (Cat.22011). Calcein Deep Red™ no puede penetrar las células vivas intactas.
Productos Relacionados
| Name | Excitation (nm) | Emission (nm) |
| Calcein Red™ AM | 562 | 576 |
| LysoBrite™ Deep Red | 597 | 619 |
Otros Productos
Bibliografia
Ver todas las 19 bibliografias: Citation Explorer
Skin-Targeted Delivery of Extracellular Vesicle-Encapsulated Curcumin Using Dissolvable Microneedle Arrays
Authors: Yerneni, Saigopalakrishna S and Yalcintas, Ezgi P and Smith, Jason D and Averick, Saadyah and Campbell, Phil G and Ozdoganlar, O Burak
Journal: Acta Biomaterialia (2022)
Radioiodination of extravesicular surface constituents to study the biocorona, cell trafficking and storage stability of extracellular vesicles
Authors: Yerneni, Saigopalakrishna S and Solomon, Talia and Smith, Jason and Campbell, Phil G
Journal: Biochimica et Biophysica Acta (BBA)-General Subjects (2022): 130069
Functional imaging of neuronal activity of auditory cortex by using Cal-520 in anesthetized and awake mice
Authors: Li, Jingcheng and Zhang, Jianxiong and Wang, Meng and Pan, Junxia and Chen, Xiaowei and Liao, Xiang
Journal: Biomedical Optics Express (2017): 2599–2610
NINJ2–A novel regulator of endothelial inflammation and activation
Authors: Wang, Jingjing and Fa, Jingjing and Wang, Pengyun and Jia, Xinzhen and Peng, Huixin and Chen, Jing and Wang, Yifan and Wang, Chenhui and Chen, Qiuyun and Tu, Xin and others, undefined
Journal: Cellular Signalling (2017)
Influence of hypothermia and subsequent rewarming upon leukocyte-endothelial interactions and expression of Junctional-Adhesion-Molecules A and B
Authors: Bogert, Nicolai V and Werner, Isabella and Kornberger, Angela and Meybohm, Patrick and Moritz, Anton and Keller, Till and Stock, Ulrich A and Beiras-Fern, undefined and ez, Andres
Journal: Scientific reports (2016)
Inhibition of ABC transport proteins by oil sands process affected water
Authors: Alharbi, Hattan A and Saunders, David MV and Al-Mousa, Ahmed and Alcorn, Jane and Pereira, Alberto S and Martin, Jonathan W and Giesy, John P and Wiseman, Steve B
Journal: Aquatic Toxicology (2016): 81–88
Rapid generation of collagen-based microtissues to study cell–matrix interactions
Authors: Brett, Marie-Elena and Crampton, Alex and ra L , undefined and Wood, David K
Journal: Technology (2016): 1–8
Toxicokinetics and toxicodynamics of chlorpyrifos is altered in embryos of Japanese medaka exposed to oil sands process-affected water: evidence for inhibition of P-glycoprotein
Authors: Alharbi, Hattan A and Alcorn, Jane and Al-Mousa, Ahmed and Giesy, John P and Wiseman, Steve B
Journal: Journal of Applied Toxicology (2016)
Flexible Endoscopic Spray Application of Respiratory Epithelial Cells as Platform Technology to Apply Cells in Tubular Organs
Authors: Thiebes, Anja Lena and Reddemann, Manuel Armin and Palmer, Johannes and Kneer, Reinhold and Jockenhoevel, Stefan and Cornelissen, Christian Gabriel
Journal: Tissue Engineering Part C: Methods (2016): 322–331
Erythropoietin Stimulates Endothelial Progenitor Cells to Induce Endothelialization in an Aneurysm Neck After Coil Embolization by Modulating Vascular Endothelial Growth Factor
Authors: Liu, Peixi and Zhou, Yingjie and An, Qingzhu and Song, Yaying and Chen, Xi and Yang, Guo-Yuan and Zhu, Wei
Journal: MEDICINE (2016): 1–8
Referencias
Ver todas las 84 referencias: Citation Explorer
Functional evidence that the self-renewal gene NANOG regulates esophageal squamous cancer development
Authors: Li, Deng and Xiang, Xiaocong and Yang, Fei and Xiao, Dongqin and Liu, Kang and Chen, Zhu and Zhang, Ruolan and Feng, Gang
Journal: Biochemical and Biophysical Research Communications (2017)
Localized functional chemical stimulation of TE 671 cells cultured on nanoporous membrane by calcein and acetylcholine
Authors: Zibek S, Stett A, Koltay P, Hu M, Zengerle R, Nisch W, Stelzle M.
Journal: Biophys J. (2006)
A vaccination and challenge model using calcein marked fish
Authors: Klesius PH, Evans JJ, Shoemaker CA, Pasnik DJ.
Journal: Fish Shellfish Immunol (2006): 20
Novel fluorescence assay using calcein-AM for the determination of human erythrocyte viability and aging
Authors: Bratosin D, Mitrofan L, Palii C, Estaquier J, Montreuil J.
Journal: Cytometry A (2005): 78
Cytotoxic effects of 100 reference compounds on Hep G2 and HeLa cells and of 60 compounds on ECC-1 and CHO cells. I mechanistic assays on ROS, glutathione depletion and calcein uptake
Authors: Schoonen WG, Westerink WM, de Roos JA, Debiton E.
Journal: Toxicol In Vitro (2005): 505
Calcein AM release-based cytotoxic cell assay for fish leucocytes
Authors: Iwanowicz LR, Densmore CL, Ottinger CA.
Journal: Fish Shellfish Immunol (2004): 127
Calcein-AM is a detector of intracellular oxidative activity
Authors: Uggeri J, Gatti R, Belletti S, Sc and roglio R, Corradini R, Rotoli BM, Orl and ini G., undefined
Journal: Histochem Cell Biol (2004): 499
Comparison of the usefulness of the MTT, ATP, and calcein assays to predict the potency of cytotoxic agents in various human cancer cell lines
Authors: Mueller H, Kassack MU, Wiese M.
Journal: J Biomol Screen (2004): 506
In vitro assay of mineralized-tissue formation on titanium using fluorescent staining with calcein blue
Authors: Goto T, Kajiwara H, Yoshinari M, Fukuhara E, Kobayashi S, Tanaka T.
Journal: Biomaterials (2003): 3885
The effects of calcium chloride and sodium chloride on the electroporation-mediated skin permeation of fluorescein isothiocyanate (FITC)-dextrans in vitro
Authors: Tokudome Y, Sugibayashi K.
Journal: Biol Pharm Bull (2003): 1508
Application notes
Experimental Protocol for Calcein AM Assay
Annexin V
Calcein
Calcein AM Assay Standardization
Multi-Color Labeling and Functional Analysis of Live Cells Using Fluorescent Calcein AM Dyes
FAQ
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there upgraded trypan blue derivatives for cell viability testing?
Is Calcein, AM toxic to cells?
What are common laser lines used in flow cytometry?
What does trypan blue do?
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