Sustrato de peroxidasa Azul Amplite®

Amplite Blue

Sustrato de peroxidasa Azul Amplite® permite realizar ensayos de detección rápida de HRP con mayor sensibilidad que los sustratos colorimétricos de HRP (como TMB y OPD).


Sustrato de peroxidasa Azul Amplite®

Hay una variedad de sustratos disponibles para detectar la actividad de la peroxidasa en los ensayos ELISA. Los sustratos colorimétricos de HRP (p. ej., TMB, OPD y ABTS) se han utilizado ampliamente durante años. Cada uno de estos sustratos varía mucho con respecto a sus características de rendimiento, como la sensibilidad de detección, el rango de trabajo y las relaciones señal/ruido alcanzables.

Nuestro sustrato de peroxidasa Amplite® Blue es fluorogénico soluble para la detección de la actividad de la peroxidasa. Amplite® Blue permite realizar ensayos de detección rápida de HRP con mayor sensibilidad que los sustratos colorimétricos de HRP (como TMB y OPD). El producto fluorescente de Amplite® Blue no fotoblanquea y exhibe una línea de base plana en los ensayos, lo que facilita la sensibilidad de detección de bajo nivel y permite altas relaciones señal-ruido.

Nombre en Ingles: Amplite® Blue peroxidase substrate

AAT-11005Sustrato de peroxidasa Azul Amplite®25 mg

Importante, Solo para uso en investigación (RUO). Almacenamiento: Congelación a <-15 °C. Minimizar la exposición a la luz.


Lector de Microplacas de Flourescencia

Excitación324 nm
Emisión409 nm
Cuttoff350 nm
Placa recomendada Placa negra

Notas importantes
Es importante almacenar a <-15 °C y debe almacenarse en un lugar fresco y oscuro.

Se puede utilizar en un plazo de 12 meses a partir de la fecha de recepción.


Figura 1. La respuesta a la dosis de HRP se midió con el kit de ensayo de peroxidasa fluorimétrica Amplite® en una placa negra utilizando un lector de microplacas de fluorescencia Gemini (Molecular Devices).

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