[6-Carboxy-2′,4,4′,5′,7,7′-hexachlorofluorescein, succinimidyl ester] *Single Isomer*
Descripción
6-HEX, SE es el éster succinimidilo reactivo con amina del ácido 6-HEX. Es ampliamente utilizado en la secuenciación de ácidos nucleicos e investigaciones relacionadas.
| Catalogo | Producto | Presentacion |
|---|---|---|
| AAT-202 | 6-HEX, SE [6-Carboxy-2′,4,4′,5′,7,7′-hexachlorofluorescein, succinimidyl ester] *Single Isomer* | 5mg |
Importante: Solo para uso en investigación (RUO). Almacenamiento: Congelacion (< -15 °C). Minimizar la exposición a la luz.
Propiedades fisicas
| Peso Molecular | 680.06 |
| Disolvente | DMSO |
Espectro
Abrir en Advanced Spectrum Viewer
Propiedades Espectrales
| Factor de Corrección (280 nm) | 0.15 |
| Coeficiente de Extinción (cm -1 M -1) | 73000 |
| Excitación (nm) | 533 |
| Emisión (nm) | 559 |
Calculadora
Preparación de la solución de stock común
Volumen de DMSO necesario para reconstituir la masa específica de 6-HEX, SE [6-Carboxy-2′,4,4′,5′,7,7′-hexaclorofluoresceína, succinimidil éster] *Isómero único* a la concentración dada . Tenga en cuenta que el volumen es solo para preparar la solución madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales/fisiológicos apropiados.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 147.046 µL | 735.229 µL | 1.47 mL | 7.352 mL | 14.705 mL |
| 5 mM | 29.409 µL | 147.046 µL | 294.092 µL | 1.47 mL | 2.941 mL |
| 10 mM | 14.705 µL | 73.523 µL | 147.046 µL | 735.229 µL | 1.47 mL |
Estructura química de 6-HEX, SE [6-Carboxy-2′,4,4′,5′,7,7′-hexaclorofluoresceína, succinimidil éster] *Isómero único*
Productos Similares
| Nombre | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
| 6-FAM, SE [6-Carboxyfluorescein, succinimidyl ester] *CAS 92557-81-8* | 493 | 517 | 83000 | 0.178 |
| 6-JOE, SE [6-Carboxy-4′,5′-dichloro-2′,7′-dimethoxyfluorescein, succinimidyl ester] *CAS#: 113394-23-3* | 520 | 545 | 750001 | – |
| 6-TET, SE [6-Carboxy-2′,4,7′,7-tetrachlorofluorescein, succinimidyl ester] | 521 | 542 | 73000 | 0.13 |
| 6-HEX azide | 533 | 559 | 73000 | 0.15 |
| 6-HEX alkyne | 533 | 559 | 73000 | 0.15 |
| 6-CR6G, SE [6-Carboxyrhodamine 6G, succinimidyl ester] *Single isomer* | 522 | 546 | 94000 | 0.214 |
| 6-TAMRA, SE [6-Carboxytetramethylrhodamine, succinimidyl ester] *CAS#: 150810-69-8* | 552 | 578 | 90000 | 0.178 |
| 6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester] *CAS#: 216699-36-4* | 578 | 604 | 82000 | 0.168 |
| 6-HEX phosphoramidite [5′-Hexachlorofluorescein phosphoramidite] | 533 | 559 | 73000 | 0.15 |
Bibliografía
DNaasikontaminaation m{\”a}{\”a}ritysmenetelm{\”a}n karakterisointi
Authors: Sn{\”a}ll, Juho
Journal: (2019)
Referencias
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Application Notes (en Ingles)
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)