{"id":2897,"date":"2023-01-06T16:36:47","date_gmt":"2023-01-06T22:36:47","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=2897"},"modified":"2023-01-20T16:59:27","modified_gmt":"2023-01-20T22:59:27","slug":"cell-meter-apoptotic-and-necrotic-multiplexing-detection-kit-triple-fluorescence-colors","status":"publish","type":"al_product","link":"https:\/\/www.cidsamexico.com\/index.php\/productos\/cell-meter-apoptotic-and-necrotic-multiplexing-detection-kit-triple-fluorescence-colors\/","title":{"rendered":"Cell Meter\u2122 Apoptotic and Necrotic Multiplexing Detection Kit *Triple Fluorescence Colors*"},"content":{"rendered":"\n

Nuestros kits de ensayo Modelo Cell Meter\u2122 son un conjunto de herramientas para monitorear la viabilidad celular. Hay una variedad de par\u00e1metros que se pueden utilizar. Este kit en particular est\u00e1 dise\u00f1ado para monitorear c\u00e9lulas apopt\u00f3ticas, necr\u00f3ticas y sanas. La apoptosis se describe como un proceso activo y programado de desmantelamiento celular aut\u00f3nomo que evita provocar inflamaci\u00f3n. En la apoptosis, la fosfatidilserina (PS) se transfiere a la membrana externa de la membrana plasm\u00e1tica. Como indicador universal de las etapas inicial\/intermedia de la apoptosis celular, la aparici\u00f3n de fosfatidilserina en la superficie celular puede detectarse antes de que se observen los cambios morfol\u00f3gicos. El sensor PS utilizado en este kit tiene fluorescencia verde al unirse a la PS de la membrana. <\/p>\n\n\n\n

La necrosis se ha caracterizado como muerte celular pasiva y accidental que resulta de perturbaciones ambientales con liberaci\u00f3n incontrolada de contenidos celulares inflamatorios. La membrana impermeable pierde su integridad demostrado por la tinci\u00f3n del n\u00facleo con 7-AAD  (Ex\/Em = 546\/647 nm), esto representa un enfoque sencillo para demostrar la apoptosis y la necrosis en etapa tard\u00eda. Adem\u00e1s, este kit proporciona un colorante para marcar el  citoplasma de c\u00e9lulas vivas CytoCalcein\u2122 Violet 450 (Ex\/Em = 405\/450 nm). <\/p>\n\n\n\n

Este kit est\u00e1 optimizado para detectar apoptosis celular (verde), necrosis (verde y\/o rojo) y c\u00e9lulas sanas (azul) con un cit\u00f3metro de flujo y un microscopio de fluorescencia.  <\/p>\n\n\n\n

<\/div>\n\n\n\n
Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-22840<\/td>Cell Meter\u2122 Apoptotic and Necrotic Multiplexing Detection Kit *Triple Fluorescence Colors*<\/td>100 pruebas<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

\"pdf\"SDS<\/a>\"pdf\"Protocol<\/a><\/p>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). <\/p>\n\n\n\n

<\/div>\n\n\n\n

Plataforma<\/mark><\/p>\n\n\n\n

Cit\u00f3metro de flujo<\/mark> <\/p>\n\n\n\n

Excitaci\u00f3n<\/td>405 nm, 488 nm laser<\/td><\/tr>
Emisi\u00f3n<\/td>450\/40 nm, 530\/30 nm, 670\/14 nm filter<\/td><\/tr>
Especificaciones instrumento<\/td>Pacific Blue, FITC, canal PE-Cy5 <\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Microscopio de Fluorescencia <\/mark><\/p>\n\n\n\n

Excitaci\u00f3n<\/td>DAPI, FITC, filtro Texas Red <\/td><\/tr>
Emisi\u00f3n<\/td>DAPI, FITC, filtro Texas Red <\/td><\/tr>
Placa recomendada<\/td>Pared negra, fondo claro<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

<\/p>\n\n\n\n

Componentes<\/mark><\/p>\n\n\n\n

Componente A: 100X Apopxin\u2122 Green<\/td>1 vial (200 \u00b5L)<\/td><\/tr>
Componente B: Buffer de ensayo<\/td>1 botella (50 mL)<\/td><\/tr>
Componente C: 200X 7-AAD<\/td>1 vial (100 \u00b5L)<\/td><\/tr>
Componente D: CytoCalcein\u2122 Violet 450<\/td>1 vial (polvo liofilizado)<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n
\n

PREPARACION DE SOLUCIONES DE STOCK<\/p>\n\n\n\n

A menos que se indique lo contrario, todas las soluciones madre no utilizadas deben dividirse en al\u00edcuotas de un solo uso y almacenarse a -20 \u00b0C despu\u00e9s de la preparaci\u00f3n. Evite los ciclos repetidos de congelaci\u00f3n y descongelaci\u00f3n. <\/p>\n\n\n\n

  1. Soluci\u00f3n madre CytoCalcein\u2122 Violet 450 (200X):<\/em>
    Agregue 100 \u00b5L de DMSO en el vial de CytoCalcein\u2122 Violet 450 (Componente D) para preparar una soluci\u00f3n madre de CytoCalcein\u2122 Violet 450 200X. Proteger de la luz.<\/li><\/ol>\n<\/div><\/div>\n\n\n\n
    <\/div>\n\n\n\n

    Para una guia sobre la preparaci\u00f3n de muestras de c\u00e9lulas, visite
    https:\/\/www.aatbio.com\/resources\/guides\/cell-sample-preparation.html<\/a><\/p>\n\n\n\n

    <\/div>\n\n\n\n

    Imagenes<\/mark><\/p>\n\n\n\n

    \"\"
    Fig. 1<\/figcaption><\/figure>\n\n\n\n

    Figura 1.<\/strong> Las im\u00e1genes de fluorescencia que muestran c\u00e9lulas vivas (azul, te\u00f1idas con CytoCalcein\u2122 Violet 450), apopt\u00f3ticas (verde, te\u00f1idas con Apopxin\u2122 Green) y necr\u00f3ticas (rojas, indicadas por tinci\u00f3n con 7-AAD) en c\u00e9lulas Jurkat inducidas por 1 \u00b5M de Estaurosporina durante 3 horas. Las im\u00e1genes de fluorescencia de las c\u00e9lulas se tomaron con un microscopio de fluorescencia Olympus a trav\u00e9s del canal Violet, FITC y Texas Red respectivamente. Las im\u00e1genes individuales tomadas de cada canal de la misma poblaci\u00f3n celular se fusionaron como se muestra arriba. Izquierda: c\u00e9lulas de control no inducidas; Derecha: tinci\u00f3n triple de c\u00e9lulas inducidas por estaurosporina.<\/p>\n\n\n\n

    <\/div>\n\n\n\n
    \"\"
    Fig. 2<\/em><\/figcaption><\/figure>\n\n\n\n

    Figura 2. <\/strong>La detecci\u00f3n de la actividad de uni\u00f3n de Apopxin\u2122 Green a la fosfatidilserina en c\u00e9lulas Jurkat. Las c\u00e9lulas Jurkat se trataron sin (A. Blue) o con estaurosporina 1 \u03bcM (A. Red) en una incubadora de CO2 a 37o<\/sup>C, al 5 % durante 5 horas y luego se cargaron con Apopxin\u2122 Green durante 30 minutos. La intensidad de fluorescencia de Apopxin\u2122 Green se midi\u00f3 con un cit\u00f3metro de flujo FACSCalibur (Becton Dickinson) utilizando el canal FL1.<\/p>\n\n\n\n

    <\/div>\n\n\n\n

    Producto Relacionado<\/mark><\/p>\n\n\n\n

    Nombre<\/td><\/tr>
    Cell Meter\u2122 Apoptotic and Necrotic Multiplexing Detection Kit II *Triple Fluorescence Colors*<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
    <\/div>\n\n\n\n

    Bibliograf\u00eda<\/mark><\/mark><\/p>\n\n\n\n

    Transcriptional coregulator Ess2 controls survival of post-thymic CD4+ T cells through the Myc and IL-7 signaling pathways<\/a>
    Authors: <\/strong>Takada, Ichiro and Hidano, Shinya and Takahashi, Sayuri and Yanaka, Kaori and Ogawa, Hidesato and Tsuchiya, Megumi and Yokoyama, Atsushi and Sato, Shingo and Ochi, Hiroki and Nakagawa, Tohru and others,
    Journal: <\/strong>Journal of Biological Chemistry (2022)<\/p>\n\n\n\n

    Acrylonitrile Butadiene Styrene (ABS) and Polycarbonate (PC) Filaments Three-Dimensional (3-D) Printer Emissions-Induced Cell Toxicity<\/a>
    Authors: <\/strong>Farcas, Mariana T and Stefaniak, Aleks and r B , undefined and Knepp, Alycia K and Bowers, Lauren and M, undefined and ler, William K and Kashon, Michael and Jackson, Stephen R and Stueckle, Todd A and Sisler, Jenifer D and Friend, Sherri A and others, undefined
    Journal: <\/strong>Toxicology Letters (2019)<\/p>\n\n\n\n

    Anthocyanin-rich blackcurrant extract inhibits proliferation of the MCF10A healthy human breast epithelial cell line through induction of G0\/G1 arrest and apoptosis<\/a>
    Authors: <\/strong>Nanashima, Naoki and Horie, Kayo and Chiba, Mitsuru and Nakano, Manabu and Maeda, Hayato and Nakamura, Toshiya
    Journal: <\/strong>Molecular Medicine Reports (2017): 6134–6141<\/p>\n\n\n\n

    Clusterin signals via ApoER2\/VLDLR and induces meiosis of male germ cells<\/a>
    Authors: <\/strong>Riaz, Muhammad Assad and Stammler, Angelika and Borgers, Mareike and Konrad, Lutz
    Journal: <\/strong>American Journal of Translational Research (2017): 1266<\/p>\n\n\n\n

    Detecting Apoptosis, Autophagy, and Necrosis<\/a>
    Authors: <\/strong>Coleman, Jack and Liu, Rui and Wang, Kathy and Kumar, Arun
    Journal: <\/strong>Apoptosis Methods in Toxicology (2016): 77–92<\/p>\n\n\n\n

    <\/div>\n\n\n\n

    Referencias<\/mark><\/p>\n\n\n\n

    Synergistic effects and mechanisms of combined tumor necrosis factor-related apoptosis-inducing ligand and chemotherapeutic drugs or radiotherapy in killing laryngeal squamous carcinoma cells in vitro
    Authors: <\/strong>Zhang M, Zhou L.
    Journal: <\/strong>Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi (2009): 565<\/p>\n\n\n\n

    Down-regulation of myeloid cell leukemia 1 by epigallocatechin-3-gallate sensitizes rheumatoid arthritis synovial fibroblasts to tumor necrosis factor alpha-induced apoptosis
    Authors: <\/strong>Ahmed S, Silverman MD, Marotte H, Kwan K, Matuszczak N, Koch AE.
    Journal: <\/strong>Arthritis Rheum (2009): 1282<\/p>\n\n\n\n

    Agmatine protects cultured retinal ganglion cells from tumor necrosis factor-alpha-induced apoptosis
    Authors: <\/strong>Hong S, Kim CY, Lee JE, Seong GJ.
    Journal: <\/strong>Life Sci (2009): 28<\/p>\n\n\n\n

    Exaggerated up-regulation of tumor necrosis factor alpha-dependent apoptosis in the older mouse liver following reperfusion injury: targeting liver protective strategies to patient age
    Authors: <\/strong>Selzner M, Selzner N, Chen L, Borozan I, Sun J, Xue-Zhong M, Zhang J, McGilvray ID.
    Journal: <\/strong>Liver Transpl (2009): 1594<\/p>\n\n\n\n

    Oxidant stress-induced liver injury in vivo: role of apoptosis, oncotic necrosis, and c-Jun NH2-terminal kinase activation
    Authors: <\/strong>Hong JY, Lebofsky M, Farhood A, Jaeschke H.
    Journal: <\/strong>Am J Physiol Gastrointest Liver Physiol (2009): G572<\/p>\n\n\n\n

    A pharmaceutical preparation of Salvia miltiorrhiza protects cardiac myocytes from tumor necrosis factor-induced apoptosis and reduces angiotensin II-stimulated collagen synthesis in fibroblasts
    Authors: <\/strong>Ling S, Luo R, Dai A, Guo Z, Guo R, Komesaroff PA.
    Journal: <\/strong>Phytomedicine (2009): 56<\/p>\n\n\n\n

    Increased apoptosis in HepG2.2.15 cells with hepatitis B virus expression by synergistic induction of interferon-gamma and tumour necrosis factor-alpha
    Authors: <\/strong>Shi H, Guan SH.
    Journal: <\/strong>Liver Int (2009): 349<\/p>\n\n\n\n

    Outside-to-inside signaling through transmembrane tumor necrosis factor reverses pathologic interleukin-1beta production and deficient apoptosis of rheumatoid arthritis monocytes
    Authors: <\/strong>Meusch U, Rossol M, Baerwald C, Hauschildt S, Wagner U.
    Journal: <\/strong>Arthritis Rheum (2009): 2612<\/p>\n\n\n\n

    Tumor necrosis factor inhibitors block apoptosis of human epithelial cells of the salivary glands
    Authors: <\/strong>Sisto M, D’Amore M, Caprio S, Mitolo V, Scagliusi P, Lisi S.
    Journal: <\/strong>Ann N Y Acad Sci (2009): 407<\/p>\n\n\n\n

    Susceptibility of the C2 canine mastocytoma cell line to the effects of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)
    Authors: <\/strong>Elders RC, Baines SJ, Catchpole B.
    Journal: <\/strong>Vet Immunol Immunopathol (2009): 11<\/p>\n\n\n\n

    <\/div>\n\n\n\n

    Application Notes (en Ingles)<\/mark><\/p>\n\n\n\n

    Abbreviation of Common Chemical Compounds Related to Peptides<\/a>
    Annexin V<\/a>
    Calcein<\/a>
    Human High Temperature Requirement Serine Protease A1 (HTRA1) Degrades Tau Protein Aggregates<\/a>
    Abbreviation of Common Chemical Compounds Related to Peptides<\/a><\/p>\n\n\n\n

    <\/div>\n","protected":false},"excerpt":{"rendered":"

    Kit de detecci\u00f3n Cell Meter\u2122 de multiplexaci\u00f3n apopt\u00f3tica y necr\u00f3tica *Colores de fluorescencia triple*<\/p>\n","protected":false},"featured_media":2902,"template":"","al_product-cat":[34],"class_list":["post-2897","al_product","type-al_product","status-publish","has-post-thumbnail","hentry","al_product-cat-reactivos"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2897","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/www.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":9,"href":"https:\/\/www.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2897\/revisions"}],"predecessor-version":[{"id":3760,"href":"https:\/\/www.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2897\/revisions\/3760"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/2902"}],"wp:attachment":[{"href":"https:\/\/www.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=2897"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/www.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=2897"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}